Using a preparation method composed of secondary chromic acid fixation, nitrocellulose embedding and Luxol fast blue-PAS-hematoxylin stain (a discriminative staining method) for the purpose of axonal discrimination, we examined 43 human spinal cords (31 males and 12 females) at the C5 level to find the differences due to gender and aging in axons of the lateral corticospinal tract. These result...

although breast tuberculosis still remains rare, but in endemic countries should be in differential diagnosis of breast cancer and abscess. imaging modalities cannot distinguish between cancer and tuberculosis. acid fast bacilli stain; culture and pcr are helpful diagnostic methods but unfortunately are not very sensitive. negative results do not rule out this diagnosis, so it seems definite di...

The authors describe an unusual presentation of lupus vulgaris in a 5-year-old boy. The lesions had a linear arrangement with proximal spread mimicking sporotrichosis. Histopathology of the lesions revealed tuberculoid granulomas. Tubercular etiology was confirmed by the demonstration of acid fast bacilli in the smears from the regional lymph node aspirate stained with Ziehl Neelsen stain, and ...

Nyka9) employed the Gomori-Grocott's chromic acid-methenamine silver method for demonstrating tubercle bacilli in tissue sections after sublimate fixative with added hydrogen peroxide or periodic acid. He described that this procedure could demonstrate mycobacteria that had lost their acidfastness by the usual carbol fuchsin stain. Harada5) also employed the periodic acid-methenamine silver sta...

We compared the sensitivity and specificity of a modified Ziehl-Neelsen (modified-ZN) staining method for acid-fast bacilli (AFB) with that of the standard Ziehl-Neelsen (standard-ZN) staining method, using culture results with Mycobacterium tuberculosis as the "gold standard." The sensitivity (72%; 101 of 140) of the modified-ZN staining method, which uses 0.3% carbol fuchsin, was significantl...

PURPOSE To evaluate the feasibility of the interferon-gamma release assay (IGRA) as a supplementary diagnostic tool for the diagnosis of genitourinary tuberculosis (GUTB). MATERIALS AND METHODS Fifty-seven patients who were tested with the IGRA to diagnose GUTB were included. All patients had clinical or radiologic features suspicious for GUTB. Signs and symptoms included chronic dysuria with...

Isospora belli, like Cryptosporidium sp., a sporozoan parasite of the small intestine, has been isolated from both immunodeficient and immunologically normal persons. In immunocompetent persons the infection may be asymptomatic; it frequently causes mild and self-limited diarrhea. The spectrum of the disease in persons with acquired immune deficiency syndrome is not well established. I. belli d...

Granulomas were reported in 0.3% to 3% of bone marrow biopsies. The aim of the study was to evaluate the incidence and etiology of bone marrow granulomas (BMGs) in the West China Hospital, which located at a high tuberculosis (TB) prevalence area in China.A retrospective case review was performed on 11,339 bone marrow biopsies at the West China Hospital of Sichuan University between January 201...