The highly specific feulgen reaction for demonstrating desoxypentose nucleic acid has proved to be of unequal utility in the hands of bacterial cytologists for the study of the bacterial nucleus (Milovidov, 1935), and at present the principal cytochemical evidence is derived from basic staining following hydrochloric acid hydrolysis or following ribonuclease digestion. It has been generally acc...

To evaluate the importance of protein digestion rate on protein deposition, we characterized leucine kinetics after ingestion of "protein" meals of identical amino acid composition and nitrogen contents but of different digestion rates. Four groups of five or six young men received an L-[1-13C]leucine infusion and one of the following 30-g protein meals: a single meal of slowly digested casein ...

It has long been recognized that numerous dietary parameters, such as the amount and type of protein and nonprotein energy sources, affect protein metabolism. More recently, we demonstrated that the protein digestion rate is an independent factor regulating postprandial protein gain. Indeed, in young men, using a non-steady-state approach and intrinsically labeled milk protein fractions [whey p...

Treatment of the extracellular nuclease of Staphylococcus aureus, strain V8, with cyanogen bromide was previously shown to produce five polypeptides which could be arranged in a linear order. Trypsin digestion of these yielded sets of peptides, in each of which the carboxyl-terminal fragment could be identified by the absence of lysine or arginine, or by the presence of homoserine. The amino ac...

The determination of arsenic in five different extraction environments (acetic acid, EDTA, NaOH, MeOH/H2O, acetonitrile) from baby formulas sold in the market was tested. Total arsenic contents were analyzed with Flow Injection-Hydride Generation-Atomic Absorption Spectrometry (FI-HG-AAS) and Graphite Furnace-Atomic Absorption Spectrometry (GF-AAS) after digestion of samples with nitric acid us...

introduction: sugarcane (saccharum officinarum) is widely cultivated in south west of iran for production of sugar. after harvest, a lot of sugarcane tops remain on the farm that can be used to feed ruminants. the total digestible nutrients (tdn) and crude fiber of sugarcane top is about 72% and 39%, respectively. the digestibility of dry matter (54%) and crude protein (39%) of sugarcane top is...

The phosphate oxygen isotopic composition in naturally occurring particulate phosphatic compounds (y18Op) can be used as a tracer for phosphate sources and to evaluate the cycling of phosphorus (P) in the environment. However, phosphatic compounds must be converted to silver phosphate prior to isotopic analysis, a process that involves digestion of particulate matter in acid. This digestion wil...

Although it has become clear that brush border hydrolysis and consequent utilization of the monosaccharide transport system is the predominant method of absorption of the luminal products of carbohydrate digestion in man, it now appears certain that two major mechanisms are involved in the absorption of the luminal products of protein digestion; on the one hand transport of liberated free amino...

The bulk of the world's phosphate reserves is located in sedimentary horizons that contain appreciable amounts of carbonate. During beneficiation of phosphate rocks, huge amounts of oversize phosphate rocks are rejected from crushers. This reject also mostly contains low tricalcium phosphate and high carbonate content. In this study, experiments have been carried out to reduce/eliminate car...