Background: Optimization of co-cultivation parameters during Agrobacterium-mediated transformation to Oxalis corniculata evaluated were bacterial density, infection period, acetosyringone (AS) concentration and temperature. Five fold efficiency is achieved in transient gus gene expression after optimizing various parameters. The optimized conditions Agrobacterium tumefaciens growth phase A600nm...

The plant pathogen Agrobacterium tumefaciens expresses virulence (vir) genes in response to chemical signals found at the site of a plant wound. VirA, a hybrid histidine kinase, and its cognate response regulator, VirG, regulate vir gene expression. The receiver domain at the carboxyl end of VirA has been described as an inhibitory element because its removal increased vir gene expression relat...

The properties of the haem environment of an extracellular peroxidase from Pleurotus ostreatus were studied by electronic absorption spectroscopy. A high-spin ferric form was predominant in the native enzyme and a high-spin ferrous form in the reduced enzyme. Cyanide was readily bound to the haem iron in the native form, thereby changing the enzyme to a low-spin cyano adduct. The electronic abs...

Objective: This study involved the formulation of protocol of Agrobacterium rhizogenes mediated transformation for the detailed study of isoflavones (IFs) metabolism in soybean. Methods: Cotyledons were separated from 4-day-old soybean seedlings and infected with three different A. rhizogenes strains under various time durations of sonication, vacuum infiltration and cocultivated on Murashige a...

The transmembrane sensor protein VirA activates VirG in response to high levels of acetosyringone (AS). In order to respond to low levels of AS, VirA requires the periplasmic sugar-binding protein ChvE and monosaccharides released from plant wound sites. To better understand how VirA senses these inducers, the C58 virA gene was randomly mutagenized, and 14 mutants defective in vir gene inductio...

Poplar is a model system for the regeneration and genetic transformation of woody plants. To shorten the time required for studies of transgenic poplar, efforts have been made to optimize transformation methods that use Agrobacterium tumefaciens. In this study, an Agrobacterium infective suspension was treated at 4 °C for at least 10 h before infecting explants. By transforming the Populus hybr...

Abstract. Suryanti E, Rusamana I, Wahyudi AT, Akhdiya A, Sukamdjaja D. 2022. Constructing and expressing acyl-homoserine lactone lactonase (aiiA) gene for enhancing Solanum tuberosum resistance against soft rot disease. Biodiversitas 23: 1780-1787. Expression of the genes associated with pectinase production in disease Dickeya spp. are regulated by quorum sensing mechanism using acyl homoserine...

An efficient and reproducible protocol for Agrobacterium tumefaciens mediated genetic transformation was developed kodo millet (Paspalum scrobiculatum L.) by optimizing various parameters. strains EHA 105 LBA 4404 harboring plasmids pCNL 56 pCAMBIA 2300, respectively, provided the highest efficiency. Addition of acetosyringone (AS) in infection medium (200 µM-EHA 105, 250 µM-LBA 4404) co-cultiv...