Here we describe a straightforward, efficient, and reliable way to clone an insert of choice into a plasmid of choice without restriction endonucleases or T4 DNA ligase. Chimeric primers containing plasmid sequence at the 5' ends and insert sequence at the 3' ends were used to PCR-amplify insertion sequences of various sizes, namely the genes for GFP (gfp), beta-d-glucuronidase (gusA), and beta...

Kidney tumors from stilbene estrogen (diethylstilbestrol)-treated Syrian hamsters were screened for somatic genetic alterations by Random Amplified Polymorphic DNA-polymerase chain-reaction (RAPD-PCR) fingerprinting. Fingerprints from tumor tissue were generated by single arbitrary primers and compared with fingerprints for normal tissue from the same animal, as well as normal and tumor tissues...

OBJECTIVE To develop a rapid and definite diagnostic test of bacterial enteritis caused by pathogenic enterobacteria, the most frequent etiologic agent of infectious enteritis in the world. METHODS A set of conventional PCR assays were applied to detect and identify salmonella, shigella, and E. coli O157:H7 directly from pure culture and fecal samples. The general primers of pathogenic entero...

saffron (crocus sativus) is the most valuable and indigenous crop in iran. the stigmas of flower are used as a popular natural flavouring, colouring and medicinal agent. however, the market suffers from frauds in this plant such as mixing with safflower petals due to high profit. identification of these frauds with conventional and biochemical methods is difficult and low sensitive. therefore, ...

In this study, we report a simple procedure for developing and using new types of polymerase chain reaction (PCR) primers, named ‘‘high-frequency oligonucleotides–targeting active genes’’ (HFO-TAG). The HFOTAG primers were constructed by first using a ‘‘practical extraction and report language’’ script to identify oligonucleotides (8, 9, and 10 bases) that exist in high frequency in 4700 expres...

genetic diversity relationships of 50 isolates of cytospora schulzeri on apple from different parts of the semirom region were analyzed using 15 polymerase chain reaction (pcr) based markers, 7 random amplified polymorphic dnas (rapds) and 8 microsatellite primed polymerase chain reaction (mp-pcr). using 7 selected rapd primers 113 bands were generated, of which 81 bands were polymorphic (71.7%...

There is a lack of information on the molecular characterization of Ocimum species and hence, efforts have been made under the present study to characterize 17 Ocimum genotypes belonging to 5 different species (O. basilicum, O. americanum, O. sanctum, O. gratissimum and O. Polystachyon) through random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. PCR amplifi...

Fusarium verticillioides (F. verticillioides) is not only a primary pathogen of maize, but also can causedisease in other crops such as sorghum. Pathogenicity is related to mycotoxin production such as fumonisin.In the present study, 24 isolates of F. verticillioides, which were previously identified by phenotype basedmethods, were re-identified using restriction fragment length polymorphism (R...