Background: Magnetic separation using magnetic nanoparticles can be used as a simple method to isolate desulfurizing bacteria from a biphasic oil/water system. Objectives: Magnetite nanoparticles were applied to coat the surface of Rhodococcus erythropolis IGTS8 and Rhodococcus erythropolis FMF desulfurizing bacterial cells, and the viability and...

Background: Classical bacteriological detection of Brucella species from food, and environment is routinely carried out based on morphological and biochemical characteristics. However, for increasing specificity and sensitivity of species identification methods, development of a molecular assay is necessary that was main aim of this study. Methods: Panel of some reference strains belonging to ...

The antibacterial activity of Citrus karna fruit peel extracts was evaluated on bacteria strains like Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. Hexane, chloroform and methanol were used for extraction and the antibacterial activity was performed by agar well diffusion method. The results were compared with ampicillin, a commercial antibacterial agent...

Escherichia coli K-12 Hfr and F- strains were successively implanted in axenic chicks. Conjugation with exchanges of chromosomal genes occurred with high frequencies in the gut of the chicks and could continue as long as fertile strains coexisted in this environment. Almost all of the expected recombinant types were recovered in the feces under these experimental conditions. Furthermore, these ...

Endophytic and plant-associated bacteria were isolated from plants and rhizoplane soil of naturally grown Brachiaria grasses at International Livestock Research Institute in Nairobi, Kenya. Eighty-four bacterial strains were isolated from leaf tissues, root tissues, and rhizoplane soil on nutrient agar and 869 media. All bacterial strains were identified to the lowest possible taxonomic unit us...

The fluorogenic acetylesterase (acetic ester hydrolase EC 3.1.1.6.) substrate, fluorescein diacetate, was used to measure enzyme activity in living protist cells. The visual enzyme assay was done by monitoring fluorochromasia by fluorescent microscopy. Quantitative fluorogenic assays were done by measuring the evolved fluorescein in a fluorometer. Of 59 strains of bacteria, 35 were fluorochroma...

the gram-negative bacterium xenorhabdus is mutualistically associated with entomopathogenic nematode, steinernema. three bacterial strains were isolated from the entomopathogenic nematodes, steinernema glaseri (steiner) and s. carpocapsae (weiser), of the white grubs, polyphylla olivieri (laporte de castelnau) in iran. this study was focused on the characterization of the bacterial strains by u...

This work aimed to isolate a series of bacterial strains associated with the green seaweed Ulva sp. and evaluate their capability manufacture PHA. The effect type supplemented sugars found be in macroalgae, on growth PHA productivity was studied. Analysis 16S rRNA gene sequence isolated revealed that PHA-producing bacteria were phylogenetically related genus Cobetia, Bacillus, Pseudoaltermonas ...

Pure culture experiments were conducted to assess the bacteriostatic and bactericidal effects of phlorotannins (PT) isolated from Ascophyllum nodosum (brown seaweed) on Escherichia coli O157:H7. In Exp. 1, one non-O157:H7 strain (25922) and three strains of E. coli O157:H7 (3081, EDL933 and E318N) were cultured in M9 medium with PT included at 0 (control), 25, 50 or 100 μg/ml (n = 3). Bacterial...