Many of the computer science researchers now work for design automatic system for fast & accurate recognition of tuberculosis(TB), to speed up the treatment process of patient to save patient life. Any type of research is not possible without data. It is critical for computer researcher to prepare Ziehl-Neelsen stain sputum smear slide & to setup image acquisition technique on simple light micr...

The Ziehl–Neelsen (ZN) stain is important in identifying organisms that are acid fast, principally Mycobacterium tuberculosis. However, decolorisation with a weaker acid concentration (for example 1% hydrochloric acid), often used in ZN staining in histology, can result in a wider variety of organisms appearing acid fast and can be a cause of misidentification. To illustrate this point, a patie...

The authors describe an unusual presentation of lupus vulgaris in a 5-year-old boy. The lesions had a linear arrangement with proximal spread mimicking sporotrichosis. Histopathology of the lesions revealed tuberculoid granulomas. Tubercular etiology was confirmed by the demonstration of acid fast bacilli in the smears from the regional lymph node aspirate stained with Ziehl Neelsen stain, and ...

We evaluated the diagnostic performance of a Cryptosporidium immunoenzymatic assay (ELISA). Fecal samples were collected from 94 HIV-seropositive patients. All specimens were processed with a commercially-available ELISA to detect C. parvum specific coproantigen and with a modified Ziehl-Neelsen stain (ZNm) microscope exam. Overall, sensitivity of the immunoenzymatic test was 100%, with a speci...

Methods: Blood, urine, and stool samples were collected from 687 hospitalized children with diarrhoea in Basrah, Iraq. The causative agents (Bacteria and parasites) for diarrhoea were identified, especially E.coli O157: H7 which was subjected to serotyping by fluorescent technique. Direct smear; Ritchie formalin– ether–sedimentation concentration and modified Ziehl-Neelsen staining techniques w...

Cryptosporidium sp. is a zoonotic intestinal protozoa in orangutans which transmitted through contaminated water (waterborne disease). This study aims to determine infects the sumatran orangutan (Pongo abelii) and bornean pygmaeus) North Sumatera Wildlife Park. The samples used were 3 fecal from different locations, two Medan Park one Pematang Siantar Animal Samples examined using floatation me...