Western Blot tekniği, herhangi bir örnekten istenen tek proteini özgün şekilde saptamak için moleküler biyolojide kullanılan eski ve güvenilir yöntemdir. Proteinlerin uyaranlara karşı verdiği yanıtların araştırılmasında, protein ifadesinin var-yok ya da artmış-azalmış olarak değerlendirilmesinde, hücre spesifik izoformlarının belirlenmesinde, genetik hastalıkların ortaya çıkışından sorumlu prot...

background: ferritin is an iron storage protein, which plays a key role in iron metabolism. measurement of ferritin level in serum is one of the most useful indicators of iron status and also a sensitive measurement of iron deficiency. monoclonal antibodies may be useful as a tool in various aspects of ferritin investigations. in this paper, the production of a murine monoclonal antibody (mab) ...

the effects of two diets, a control diet (commercial feed with 39% crude protein) and an experimental diet (prepared based on 42% soybean meal with 38% crude protein), on growth performance of western white shrimp (litopenaeus vannamei) in six 0.4-ha-earthen ponds (three replications per treatment) with 25 per m2 density, were investigated. there was no significant difference in final weight (m...

objective(s):antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. the aim of this study was to produce polyclonal antibody against a synthetic peptide derived from n-terminal region of β-actin protein to be used as a protein loading control in western ...

background: investigation on the natural radionuclides 238u, 232th, 40k and natural fallout radionuclide 210po in the prominent plants species of western ghats tropical forest near kotagiri have been carried out as a part of baseline background radiation studies in the forest environment. materials and methods: the prominent plants species of the region evodia roxburghiana and eleaocarpus oblan...