The orphan receptor GPR17 has been reported to be activated by UDP, UDP-sugars, and cysteinyl leukotrienes, and coupled to intracellular Ca(2+) mobilization and inhibition of cAMP accumulation, but other studies have reported either a different agonist profile or lack of agonist activity altogether. To determine if GPR17 is activated by uracil nucleotides and leukotrienes, the hemagglutinin-tag...

s of Communications Meeting of the Biochemical Society Pharmacological Biochemistry Group, St. Mary3 Hospital Medical School, London 13 September 1974 GLUCURONIDATION MECHANISMS : a Colloquium organized by P. T. Nowell (Liverpool) Membrane Dependence of Uridine Diphosphate Glucuronyltransferase : Effect of the Membrane on Kinetic Properties DAVID ZAKIM and DONALD A. VESSEY Departments of Medici...

It has been previously demonstrated (1) that particulate preparations from Phase&s uureus (mung bean) seedlings are capable of catalyzing the formation of uridine diphosphate-ngalacturonic acid1 and a mixture of UDP-n-xylose and UDP-Larabinose from UDP-n-glucuronic acid. The results indicated that the preparations contained UDP-uranic acid 4-epimerase and UDP-uranic acid decarboxylase activitie...

The backfill in the stope usually forms a composite structure with surrounding rock order to bear pressure together support goaf and ensure safe mining of subsequent ores. Based on laboratory tests theoretical analysis, energy damage evolution rock–backfill materials (RBCM) are studied deeply. results show that: (1) ?p (peak stress), ?p strain), E (elasticity modulus) decreased increase interna...

Higher plants efficiently conserve energy ATP in cellulose biosynthesis by expression of sucrose synthase, in which the high free energy between glucose and fructose in sucrose can be conserved and used for the synthesis of UDP-glucose. A mixture of sucrose synthase and bacterial cellulose synthase proceeded to form UDP-glucose from sucrose plus UDP and to synthesize 1,4-beta-glucan from the su...

UDP-galactose 4-epimerase (GalE) catalyzes the interconversion of UDP-glucose (UDP-Glc) and UDP-galactose (UDP-Gal), which is a pivotal step in the Leloir pathway for d-galactose metabolism. Although GalE is widely distributed in prokaryotes and eukaryotes, little information is available regarding hyperthermophilic GalE. We overexpressed the TM0509 gene, encoding a putative GalE from Thermotog...

Neoplastic mast cells of mice (including long-established and newly derived lines) were grown in large-volume suspension cultures to provide enough cells for preparation of microsomal fractions. Microsomal preparations from P815Y and P815S cells synthesized (14)C-labelled glycosaminoglycan when incubated with UDP-[(14)C]glucuronic acid and UDP-N-acetylgalactosamine. No significant amount of (14...

This study characterized the kinetics, variability, and factors that affect UDP-glucuronic acid (UDP-GlcUA) uptake by human liver microsomes (HLM). Biphasic kinetics were observed for UDP-GlcUA uptake by HLM. Uptake affinities (assessed as Kd) of the high- and low-affinity components differed by more than an order of magnitude (13 ± 6 vs. 374 ± 175 µM), but were comparable in terms of the maxim...

The G(i)-coupled P2Y(14) receptor (P2Y(14)-R) is potently activated by UDP-sugars and UDP. Although P2Y(14)-R mRNA is prominently expressed in circulating neutrophils, the signaling pathways and functional responses associated with this receptor are undefined. In this study, we illustrate that incubation of isolated human neutrophils with UDP-glucose resulted in cytoskeleton rearrangement, chan...