UDP-glycosyltransferases (UGTs) are the most studied glycosyltransferases, and belong to large GT1 family performing key roles in antibiotic synthesis, development of bacterial glycosyltransferase inhibitors, animal inflammation. They transfer glycosyl groups from nucleotide UDP-sugars (UDP-glucose, UDP-galactose, UDP-xylose, UDP-rhamnose) acceptors including saccharides, proteins, lipids, seco...

to determine the association between polymorphism of ugt1a1 gene and idiopathic hyperbilirubinemia in iranian neonates. fifty neonates with idiopathic hyperbilirubinemia and serum total bilirubin (stb) more that 15mg/dl and 50 neonates with idiopathic hyperbilirubinemia and serum total bilirubin (stb) less than 15mg/dl enrolled in this study. thymine-adenine (ta)  repeats in the promoter region...

Sugar nucleotide–modifying enzymes of the short-chain dehydrogenase/reductase type use transient oxidation–reduction by a tightly bound nicotinamide cofactor as common strategy catalysis to promote diverse set reactions, including decarboxylation, single- or double-site epimerization, and dehydration. Although basic mechanistic principles have been worked out decades ago, finely tuned control r...

Enzymatic synthesis is a promising approach to produce hyaluronic acid (HA) products with defined molecular weight (MW) and dispersity. We here report on the evaluation of nucleoside triphosphate (NTP) regeneration system for precursors uridine-5'-diphosphate α-d-N-acetylglucosamine (UDP-GlcNAc) α-d-glucuronic (UDP-GlcA) integration in one-pot HA. By polyphosphate kinase from Ruegeria pomeroyi ...

As the world becomes increasingly connected and number of users grows exponentially “things” go online, prospect cyberspace becoming a significant target for cybercriminals is reality. Any host or device that exposed on internet prime cyberattacks. A denial-of-service (DoS) attack accountable majority these Although various solutions have been proposed by researchers to mitigate this issue, alw...

A series of nucleotide sugar interconversion enzymes (NSEs) generate the activated donors required for biosynthesis cell wall matrix polysaccharides and glycoproteins. UDP-glucose 4-epimerases (UGEs) are NSEs that function in (UDP-Glc) UDP-galactose (UDP-Gal). The roles monocots remain unclear due to redundancy pathways. Here, we report a brittle plant (bp1) rice mutant exhibits leaves culms at...

In the context of smart cities, data-driven innovation and digital transformation have received increasing attention from practitioners academics. The data-centric nature city transformations highlights essential role urban data platform (UDP) to manage large heterogeneous sets facilitate interaction among providers users in a ecosystem. To realize value creation through UDP, comprehensive unde...

This paper explores the relationship between mandate of conservation authorities and agenda community development in rural areas provides recommendations on how to improve status quo. The analysis is based qualitative data collected during five interviews with uKhahlamba Drakensberg Park’s (UDP) liaison officers traditional leaders communities surrounding UDP. results reveal disjointed coordina...

Glycyrrhetic acid 3-O-mono-β-d-glucuronide (GAMG), a rare and innovative compound in licorice, exhibits high-potency sweetness improved physiological activities. However, low amounts of GAMG from plants cannot meet the demands growing markets. In this study, an efficient one-pot multienzyme cascade reaction for biosynthesis was constructed using coupled catalysis glycosyltransferase uridine 5′-...

UDP-sugars serve as substrates in the synthesis of cell wall polysaccharides and are themselves generated through sequential interconversion reactions from UDP-Glc (UDP-glucose) as the starting substrate in the cytosol and the Golgi apparatus. For the present study, a soluble enzyme with UDP-Xyl (UDP-xylose) 4-epimerase activity was purified approx. 300-fold from pea (Pisum sativum L.) sprouts ...