One of the most important forms of preharvest damage to wheat is caused by sunn pests. The insects insert their mouth parts into the immature grain and while injecting their saliva suck the milky juices. Flour from damaged wheat results in low baking performance due to the bug proteolytic enzymes’ injected which cause the breakdown of gluten structure in the dough. In the present study three wh...

The condensation of retinene(1) with opsin to form rhodopsin is optimal at pH about 6, a pH which favors the condensation of retinene(1) with sulfhydryl rather than with amino groups. The synthesis of rhodopsin, though unaffected by the less powerful sulfhydryl reagents, monoiodoacetic acid and its amide, is inhibited completely by p-chloromercuribenzoate (PCMB). This inhibition is reversed in ...

Addition of sulfhydryl groups to the reaction mixture, to stabilize serum creatine kinase (creatine phosphokinase, CPK), results in apparent increases in the activity of this enzyme in many sera. In addition, in sera from patients just after myocard ial infarction, assays for sulfhyd ryl-activated CPK have a different clinical pattern than do those for CPK assayed without sulfhydryl activators:...

The metal cation requirements of pyruvate, phosphate dikinase from Bacteroides symbiosus have been examined with respect to both the overall reaction and the three partial reactions. The overall reaction and all three partial reactions require a divalent cation (M$‘, Mn’+, or Co’+). Although Mn2+ and Co’+ are very effective activators at low concentrations in two of the three partial reactions,...

The reactions of three organic mercurial compounds, chlormerodrin, parachloromercuribenzoate (PCMB), and parachloromercuribenzenesulfonate (PCMBS) with intact red blood cells, hemolyzed red cells, hemoglobin solutions, and hemoglobin-free ghosts have been characterized. Both PCMB and PCMBS react with only 2 to 3 sulfhydryl groups per mole of hemoglobin in solution, whereas chlormerodrin reacts ...

By investigating the sulfhydryl groups of the muscle proteins I have attempted to throw some light on the state of the proteins in muscle and on the change in state of these proteins in muscular contraction. In previous papers i t has been shown that in a denatured protein the number of active SH groups is equivalent to the quantity of cysteine found in the hydrolyzed protein (Mirsky and Anson,...

By investigating the sulfhydryl groups of the muscle proteins I have attempted to throw some light on the state of the proteins in muscle and on the change in state of these proteins in muscular contraction. In previous papers i t has been shown that in a denatured protein the number of active SH groups is equivalent to the quantity of cysteine found in the hydrolyzed protein (Mirsky and Anson,...

The contractile response of rabbit renal arteries and canine tibial arteries to prostaglandins A2, B2, F2alpha, E1, E2, D2, and B1 was associated with a reduction in total sulfhydryl group content of smooth muscle. The total sulfhydryl content of rabbit renal and canine tibial arteries and was not affected by norepinephrine or potassium chloride. Reduction of disulfide groups with dithiothreito...

The reactions of three organic mercurial compounds, chlormerodrin, parachloromercuribenzoate (PCMB), and parachloromercuribenzenesulfonate (PCMBS) with intact red blood cells, hemolyzed red cells, hemoglobin solutions, and hemoglobin-free ghosts have been characterized. Both PCMB and PCMBS react with only 2 to 3 sulfhydryl groups per mole of hemoglobin in solution, whereas chlormerodrin reacts ...