In total, 59 single Cys-replacement mutants in helix VII and helix X of the lactose permease of Escherichia coli were subjected to site-directed fluorescence labeling in right-side-out membrane vesicles to complete the testing of Cys accessibility or reactivity. For both helices, accessibility/reactivity is relatively low at the level of the sugar-binding site where the helices are tightly pack...

This paper first outlines the benefits of Medium Voltage Variable Frequency Drives (MV-VFD) compared to classical drivers or control methods (i.e. steam/gas turbines, hydraulic coupling and Direct-On-Line operation with, for example, valve/damper control). The paper then focuses on typical variable frequency drive applications in the sugar industry and also describes two real cases, namely a MV...


 Abstract:
 Consumption of milk and its products is considered as one the indicators human societies' development. Ice cream most popular around world has many fans but due to high amount sucrose negative effects on health. Stevia, a natural sweetener, was used substitute at 0-100% replacement levels in ice formulation. To investigate treatments produced, physicochemical properties i...

Stevia is a natural, non-nutritive sweetener can replace sugar in your diet to control diabetes and aid in weight loss. Naanberenji, an Iranian traditional cookie, is a well-reputed confectionary containing high sucrose and calorie value, for this reason its consumption in healthy diet has been restricted. In this study, the effect of sucrose replacement by stevioside-maltodextrin mixture on ph...

Acetate kinase catalyzes the magnesium-dependent transfer of the gamma-phosphate of ATP to acetate. The recently determined crystal structure of the Methanosarcina thermophila enzyme identifies it as a member of the sugar kinase/Hsc70/actin superfamily based on the fold and the presence of five putative nucleotide and metal binding motifs that characterize the superfamily. Residues from four of...

Following smoking cessation, individuals increase their food intake. Women experience greater postcessation hyperphagia than men, and older women may increase their food intake more than younger women. Some research has suggested that postcessation increases in food intake may be macronutrient specific. However, previous investigations of macronutrient specific changes in food intake following ...

Biochemical and biophysical studies based upon crystal structures of both a mutant and wild-type lactose permease from Escherichia coli (LacY) in an inward-facing conformation have led to a model for the symport mechanism in which both sugarand H+-binding sites are alternatively accessible to either side of the membrane. Previous findings indicate that the face of helix II with Asp68 is importa...

This work presents a technical and economic study using sugar cane bagasse ash (SCBA) to partially replace Portland cement clinker. To evaluate the viability, replacement rates of 10, 20, 30% were used in experiments. The ashes following conditions: (i) as collected (AC), (ii) ground (G), (iii) re-burnt (RG). Three composition parameters mortar mix procedures: with water factor/fixed binder vol...

BACKGROUND Acetylation of the xylan backbone was a major obstacle to enzymatic decomposition. Removal of acetyl groups by acetyl xylan esterases (AXEs) is essential for completely enzymatic hydrolysis of xylan. Appended carbohydrate binding modules (CBMs) can promote the enzymatic deconstruction of plant cell walls by targeting and proximity effects. Fungal acetyl xylan esterases are strictly a...