The serine/threonine phosphatase (PP1) isoform PP1γ2, predominantly expressed in the testis, is a key enzyme in spermatozoa. High PP1γ2 catalytic activity holds motility in check in immature spermatozoa. Inhibition of PP1γ2 causes motility initiation in immature spermatozoa and motility stimulation and changes in flagellar beat parameters in mature spermatozoa. The PP1γ2 isoform is present in a...

The aim of the study was to evaluate motility parameters of Lacaune (Lc) and east-Friesian (eF) ram frozen-thawed spermatozoa by cASA (computer-Automated Sperm Analysis) technique in relation to their in vivo fertilizing ability. Fresh ejaculates were diluted in a Triladyl extender containing 20 % egg yolk, 6 % glycerol and 0.1 % trehalose and frozen in a liquid nitrogen vapour. The evaluation ...

background: fertility protection is important in young patients with hodgkin's lymphoma. objective: the goal of this study was to determine the effects of abvd and chlvpp chemotherapeutic protocols for hodgkin's disease on the spermatozoa fertility indices of male rat. materials and methods: after determining tolerance dose of drugs in pilot study, 24 male rats were divided to four groups: abvd...

background: recently, motile sperm organelle morphology examination (msome) criteria as a new real time tool for evaluation of spermatozoa in intracytoplasmic sperm injection (icsi) cycles has been considered. objective: the aim was to investigate the predictive value of msome in in vitro fertilization (ivf) in comparison to icsi cycles and evaluation of the association between msome parameters...

Background: The aim of this study was to examine the possible relationship between sperm DNA integrity and chromatin packaging evaluated by cytochemical assays, traditional sperm parameters and recurrent spontaneous abortion (RSA) of unknown origin. Materials and Methods: In this cohort study, 40 couples with a history of RSA and 40 couples with proven fertility were considered as case and cont...

The present study was conducted to detect sperm apoptosis in fresh, capacitated and frozen ram semen and to determine its relationship with other seminal parameters as motility and viability. The cryopreservation process induces the plasma membrane disruptions, attended with increasing of phoshpatidylserine molecules expression on the extracellular surface, wherefore some of the live spermatozo...

Paternal germline exposure to organophosphorous pesticides (OP) has been associated with reproductive failures and adverse effects in the offspring. Methyl-parathion (Me-Pa), a worldwide-used OP, has reproductive adverse effects and is genotoxic to sperm, possibly via oxidative damage. This study investigated the stages of spermatogenesis susceptible to be targeted by Me-Pa exposure that impact...

The aim of this study was to develop and to test the standardized aseptic technology of permeable cryoprotectant-free vitrification of human spermatozoa in capillaries (for intracytoplasmic sperm injection [ICSI] or in vitro fertilization [IVF]). To test the effect of vitrification on basic sperm parameters, each of 68 swim-up-prepared ejaculates from oligo-astheno-terato-zoospermic patien...

Male factor infertility affects 30--40% of the subfertile couples worldwide and that poses a significant medical social issue. Different strategies are developed to increase fertilization potential male gametes during procedures assisted reproduction. One these approaches is addition biologically active molecules, which help stimulate motility spermatozoa. In current investigation, we analysed ...

Background: It has been shown that reactive oxygen species (ROS) can lead to deleterious effects on a range of sperm parameters. Vitamins E and C are capable of reducing ROS levels and lipid peroxidation. Objective: The aim of study was to determine the level of lipid peroxidation as indicated by Malondialdehyde (MDA) and vitamins E and C in sperm and seminal plasma of asthenoteratozoospermic a...