Maltooligosaccharides, up to maltooctaose, were converted to the corresponding 4-nitrophenylhydrazones and in this form effectively resolved by paper chromatography. A method of determination of maltooligosaccharides in mixtures was elaborated which includes elution of the separated 4-nitrophenylhydrazones from the paper and their spectrophotometric measurement. The analytical procedure was emp...

New multiparametric analysis and method of their evaluation were derived. The bolus dye injection is used for evaluation of ICG (indicyaningreen) and BSP (bromsulphophtalein) kinetics. This arrangement is a form of spectrophotometric measurement of BSP in plasma and/or pulse dye densitometry of ICG. Clearance values enable to differentiate liver diseases and to add their clinical staging. Patho...

A simple high-performance liquid chromatography RP-HPLC and two reproducible ultraviolet spectrophotometric methods were developed and validated for the estimation of nebivolol HCl and valsartan simultaneously in combined pharmaceutical solid dosage form. The wavelength fixed for spectrophotometric measurement of valsartan at 246.6 nm, nebivolol HCl at 280.2 nm for the simultaneous equation and...

a kinetic spectrophotometric method for rapid measurement of thiocyanate in serum, urine and sewage water with no need for removed of interfering substances in described. the method is based on the inhibitory effect of thiocyanate on the oxidation of pyrogallol red by bromated and nitrite which is monitored at 467 nm. the variables affecting the rate of the reaction were investigated and the op...

Two-component mixtures of cefsulodin and clavulanic acid were analysed by a first-derivative spectrophotometric method using a zero-crossing technique of measurement. The relative ease offered by this derivative technique for the quantification of these drugs, with closely overlapping spectral bands, was clearly demonstrated. As the absorption band of clavulanic acid closely overlaps with that ...

Int J Exerc Sci 2(2): 83-93, 2009. The measurement of blood lactate has long been used as marker of exercise intensity and training status. We compared a commercially available lactate oxidase spectrophotometric method (LO) to determine blood lactate levels to two previously validated methods, the lactate dehydrogenase spectrophotometric method (LDH), and the YSI 1500L Sport lactate analyzer (Y...

We describe a convenient, simple and novel continuous spectrophotometric method for the determination of aminoacyl-tRNA synthetase activity. The assay relies upon the measurement of inorganic pyrophosphate generated in the first step of the aminoacylation of a tRNA. Pyrophosphate release is coupled to inorganic pyrophosphatase, to generate phosphate, which in turn is used as the substrate of pu...

Problem statement: Spectrophotometric method and High-Performance Liquid Chromatography (HPLC) are the most useful tools to photosynthetic pigments analysis. The main objective of this reaserch was to compere the results obtained by these two independent methods in experiments under controlled laboratory conditions. Approach: Comparison between spectrophotometric and HPLC methods for pigment an...

One titrimetric and two spectrophotometric methods which are simple, sensitive, and economic are described for the determination of sumatriptan succinate (STS) in bulk drug and in tablet dosage form using N-bromosuccinimide (NBS) as a brominating agent. In titrimetry, aqueous solution of STS is treated with a measured excess of NBS in acetic acid medium, and after the bromination of STS is judg...