An aminopeptidase was purrned 178-fold from an extract of Grijbla frondosa by ammonium sulfate precipitation and a series of column chromatographies on pheny)-Toyopearl, Sephadex G-25, and Mono-Q. The melecular mass of the enzyme was estimated te be 27 kDa and 30kDa by gel filtration and SDS-PAGE, respectively. The enzyme had an optimum pH of 8.5 and was stable between pH 6.e and pH 10.5, and i...

Polypeptides from purified virions of the calf rotavirus (RV) isolate 993/83 and those from the pigeon RV isolate PO-13 comigrated on SDS-polyacrylamide gels. Two polypeptides of 45K and 47K were detected at the position of VP6. Both proteins behaved like authentic VP6 protein with EDTA and heat treatment. RV 993/83 and PO-13 showed identical one-dimensional peptide maps for VP2, and the 45K an...

Membrane vesicles with a high mating reactivity were obtained from cilia of Paramecium caudatum by treatment with a solution containing 2 M urea and 0.1 mM Na2-EDTA. All processes of conjugation were induced in cells of the complementary mating type by approximately 10 mug/ml proteins of the vesicles. Electron microscope observation showed that the membrane vesicles have a diameter of 100-150 n...

La producción pesquera puede generar cantidades considerables de residuos cuya disposición final inadecuada causar daños ambientales. utilización estos desechos para la obtención productos con alto valor agregado y interés industrial, es una alternativa acuícola sostenible. En este trabajo se obtuvieron caracterizaron extractos ácidos ricos en enzimas tipo pepsina (EP) alcalinos tripsina (ET) a...

Extraction of protease from a local ginger rhizome (Zingiber officinale var. Bentong) was carried out. The effect of extraction pH (6.4, 6.8, 7.0, 7.2, 7.6, 8.0, 8.4, and 8.8) and stabilizers (0.2% ascorbic acid, 0.2% ascorbic acid and 5 mM EDTA, or 10 mM cysteine and 5 mM EDTA) on protease activity during extraction was examined. pH 7.0 potassium phosphate buffer and 10 mM cysteine in combinat...

In the present study, an extracellular alkali-thermo-tolerant xylanase from Bacillus paramycoides was produced in presence of organic solvent. The enzyme purified by ammonium sulphate precipitation, gel filtration, and ion exchange chromatography, with overall recovery 25.9%. hada 70 kDa molecular weight (MW) confirmed SDS-PAGE analysis. maximum activity reported at 55 °C pH 7.0. Xylanase stabi...

Abstract Ethylenediaminetetraacetic acid disodium (EDTA) can form stable complexes with many metals. To improve the leaching rate of gold and consumption thiosulfate, EDTA ammonia were combined copper ions to a copper-EDTA-ammonia-thiosulfate system. Electrochemical methods used study effect ion, EDTA, polarization voltage. The results showed that increasing concentrations ions, promoted dissol...