The efficient preservation of protein in silage for livestock feed is dependent on the rate and extent proteolysis. Previous research fresh forage indicated enhanced stability certain Festulolium (ryegrass × fescue hybrids) cultivars compared to ryegrass. This first report an experiment test hypothesis that a Lolium perenne Festuca arundinacea var glaucescens cultivar had reduced proteolysis pe...

von Willebrand factor (VWF) multimeric composition is regulated in plasma by ADAMTS13. VWF deglycosylation enhances proteolysis by ADAMTS13. In this study, the role of terminal sialic acid residues on VWF glycans in mediating proteolysis by ADAMTS13 was investigated. Quantification and distribution of VWF sialylation was examined by sequential digestion and high-performance liquid chromatograph...

Enzymatic hydrolysis processes can be used to produce organic nutrient media from renewable raw materials. However, many of these are not optimally designed, so expensive enzymes and substrates wasted. Mathematical models Digital Twins (DTs) powerful tools, which optimize bioprocesses and, thus, increase the yield desired products. Individual enzymatic have already been modeled, but for combine...

Dystroglycan is frequently lost in adenocarcinoma. α-dystroglycan is known to become hypoglycosylated due to transcriptional silencing of LARGE, whereas β-dystroglycan is proteolytically cleaved and degraded. The mechanism and proteases involved in the cleavage events affecting β-dystroglycan are poorly understood. Using LNCaP prostate cancer cells as a model system, we have investigated protea...

We propose for the first time to divide histone proteolysis into "histone degradation" and the epigenetically connoted "histone clipping". Our initial observation is that these two different classes are very hard to distinguish both experimentally and biologically, because they can both be mediated by the same enzymes. Since the first report decades ago, proteolysis has been found in a broad sp...