Background/introduction Microbubbles (MBs) are small (1-5 μm diameter), perfluorcarbon gas filled lipid microspheres used for contrast enhancement during ultrasound imaging. MBs (Definity, others) are approved for contrast echocardiography and widely used (6 M patient injections) as ultrasound contrast agents with established pharmacokinetics and clearance data. Recent research has shifted to t...

The development of stimuli-sensitive release systems for protein and peptide delivery has been a subject of significant pharmaceutical importance in recent years, especially in relation to the possible development of intelligent delivery systems. In this review we concentrate on biological and chemical characteristics of these systems and we identify important guidelines as well as unsolved pro...

Protein therapeutics holds significant promise for improving human health [1,2]. Our organism contains thousands of proteins, which perform essential functions in growth, development and metabolism regulation. Many diseases arise from the alterations in the functions of intracellular proteins [1]. Therefore, the administration of therapeutic proteins has shown great potential in the treatment o...

There is a pressing need for long-term, controlled drug release for sustained treatment of chronic or persistent medical conditions and diseases. Guided drug delivery is difficult because therapeutic compounds need to survive numerous transport barriers and binding targets throughout the body. Nanoscale protein-based polymers are increasingly used for drug and vaccine delivery to cross these bi...

Aim of this work was to compare the efficacy of two loading methods of proteins onto polymeric nanocarriers: adsorption and encapsulation methods. Preliminary studies of protein loading were done using Bovine Serum Albumin (BSA) as model protein. Nanocarriers were prepared starting from polylactic co-glycolic acid (PLGA) polymer; production methods used are two different variants of emulsion ev...

background: balloon bionic midwifery has been applied in clinical obstetrics in china for 10 years, although played a certain role in controlling and improving the quality of obstetrics and caesarean section rate, but some questions have not been resolved. objective: the aim of this study was to investigate the efficacy and safety of airbag midwifery. materials and methods: primiparas (2410 cas...

In this study, in order to facilitate and accelerate the production of eukaryotic protein alpha 1-antitrypsin (AAT) with correct post-translational modifications, a protein production system based on the transduction of CHO and COS-7 cells using lentiviral vectors was developed. Human AAT cDNA was cloned into a replication-defective lentiviral vector. The transgene AAT-Jred chimer was transferr...

Objective(s): P-glycoprotein (P-gp) is an efflux protein, the overexpression of which has been associated with multidrug resistance in various cancers. Although siRNA delivery to reverse P-gp expression may be promising for sensitizing of tumor cells to cytotoxic drugs, the therapeutic use of siRNA requires effective carriers that can deliver siRNA intracellularly with minimal toxicity on targe...