Ayu (Plecoglossus altivelis), one kinds of valuable cultured fish species, show almost no morphological difference between male and female until sexual maturity. Here, we report the identification of sex-linked markers for the ayu, based on Amplified Fragment Length Polymorphism (AFLP) generated from cultured fish (15 males and 15 females) by using 63 different primer combinations. Genomic frag...

Three Iranian native strains and three Japanese commercial lines of the silkworm Bombyx mori were analyzed using amplified fragment length polymorphism (AFLP) markers. A set of ten PstI/TaqI primer combinations amplified a total of 322 bands out of which 251 (%78) were polymorphic. Estimates of Nei’s gene diversity for all loci in individual strains and commercial lines indicated a higher degre...

to identify qtls controlling grain yield and yield components, an f2 population consisting of 188 plants derived from the cross between two rice pure lines, binam and kadous, were grown in research field of faculty of agricultural sciences, university of guilan, rasht, iran, in 2008, and were recorded for 9 important traits related to grain yield. to construct linkage map, 85 marker of ssrs and...

Objective: To evaluate the effect of different metal primers and surface treatment methods on the bond strength of 2 resin cements to yttrium-stabilized tetragonal zirconia (Y-TZP) ceramic material. Methods: 240 cylindrical dentin specimens were cut from extracted human third molars. The same number of zirconia ceramic (Cercon) plates were made and assigned to 24 groups based on combinations of...

UNLABELLED PREMISE OF THE STUDY We report 11 primer sets for nine single-copy nuclear genes in Streptanthus and other Thelypodieae (Brassicaceae) and their utility at tribal-level and species-level phylogenetics in this poorly resolved group. • METHODS AND RESULTS We selected regions based on a cross-referenced matrix of previous studies and public Brassica expressed sequence tags. To desi...

A polymerase chain reaction (PCR) primer sequence is called degenerate if some of its positions have several possible bases. The degeneracy of the primer is the number of unique sequence combinations it contains. We study the problem of designing a pair of primers with prescribed degeneracy that match a maximum number of given input sequences. Such problems occur, for example, when studying a f...

W.C. RICE. 1999. A PCR-based method was developed for the rapid detection of vip3A gene encoding a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects. Specific primer combinations (three primers for the normal strand and two primers for the complementary strand) were capable of generating diagnostic fragments that success...

The present study was conducted to investigate genetic diversity between and within of six populations with different individual numbers of cultivated and non-cultivated provenances of  Moringa peregrina (Forssk.) Fiori and Moringa oleifera Lam. using RAPD, ISSR and various combinations of RAPD and ISSR primers in one multiplex PCR (R-ISSR). 10 primers that produced clear and ...

Eighteen sequence-related amplified polymorphism (SRAP) primer combinations were used to assess the genetic diversity of 126 individuals from five different geographical populations of Vitis ficifolia Bge. The numbers of bands scored per primer combination ranged from 8 to 27, with an average of 18.6 bands. At the population level, the percentage of polymorphic bands (PPB), Nei's gene diversity...