BACKGROUND The aim of this study was to evaluate fibroblast co-culture on in vitro maturation and fertilization of prepubertal mouse preantral follicles. MATERIALS AND METHODS The ovaries of 12-14 day old mice were dissected and 120-150 μm intact preantral follicles with one or two layers of granulosa cells, and round oocytes were cultured individually in α-minimal essential medium (α-MEM) su...

background: one of the most major obstacles of ovarian tissue vitrification is suboptimal developmental competence of follicles. matrix metalloproteinases 2 (mmp-2) and 9 (mmp-9) and their tissue inhibitors timp-1 and timp-2 are involved in the remodeling of the extracellular matrix in the ovaries. objective: this study aimed to evaluate the expression of mmp-2, mmp-9, timp-1, and timp-2 genes ...

The TGFβ superfamily comprises several bone morphogenetic proteins (BMP) capable of exerting gonadotropin-independent effects on the development of small preantral follicles. In embryonic tissues, BMP concentration gradients, partly formed by antagonistic factors, are essential for establishing phenotypic fate. By examining the expression of candidate genes whose protein products are known to i...

Mammalian females are born with a finite number of nonrenewing primordial follicles, the majority of which remain in a quiescent state for many years. Because of their nonrenewing nature, these "resting" oocytes are particularly vulnerable to xenobiotic insult, resulting in premature ovarian senescence and the formation of dysfunctional oocytes. In this study, we characterized the mechanisms of...

STUDY QUESTION What are the effects of exposure of ovarian tissue to dehydroepiandrosterone (DHEA) supplementation in vivo? SUMMARY ANSWER DHEA exposure stimulates initiation of primordial follicles and development of gonadotrophin-responsive preantral/early antral follicles possibly mediated through promoting granulosa cell proliferation and enhancing anti-Mullerian hormone (AMH) expression....

To compare the efficiency of North Carolina State University medium 23 (NCSU23) and Alpha Minimum Essential Medium (?-MEM) as a base medium, to evaluate effects Anti-Müllerian Hormone (AMH) alone or in combination with Follicle Stimulating (FSH) on vitro development steroid production isolated porcine preantral follicles. Porcine secondary follicles were cultured NCSU23 ?-MEM media for 4 days. ...

Background: The majority of thousands of oocytes in the ovaries are small, non-growing and reside in preantral follicles (PFs). The development of a culture system for preantral follicle may be very useful for understanding the complex mechanism in folliculogenesis at early stages of development as wells as could offer a significant way for the propagation of livestock, including goats. This pa...