the objective of this research was to investigate the influence of the over-expression of recombinant interferon-g during high cell density cultivation on cellular characteristics of recombinant e. coli. batch and fed-batch culture techniques were employed to grow escherichia coli bl21 for production of human gamma-interferon in pet expression system. final cell densities in batch and fed-batch...

Plasmids 1.6, 2.8, or greater than 40 megadaltons in size were found in one urethral and nine throat strains of meningococci. Throat meningococci are known to be heterogeneous in their aminopeptidase profiles. Their unexpected content of plasmids is further evidence of their difference from classic systemic strains. Although the 2.8 megadalton plasmid has some resemblance to the well known 2.6 ...

We analyzed the inheritance of mitochondrial DNA (mtDNA) species in matings of the grass symbiont Epichloë typhina. Eighty progeny were analyzed from a cross in which the maternal (stromal) parent possessed three linear plasmids, designated Callan-a (7.5 kb), Aubonne-a (2.1 kb) and Bergell (2.0 kb), and the paternal parent had one plasmid, Aubonne-b (2.1 kb). Maternal transmission of all plasmi...

The Yeast Knockout (YKO) collection has provided a wealth of functional annotations from genome-wide screens. An unintended consequence is that 76% of gene annotations derive from one genotype. The nutritional auxotrophies in the YKO, in particular, have phenotypic consequences. To address this issue, 'prototrophic' versions of the YKO collection have been constructed, either by introducing a p...

ABSTRACT We conducted a comparative analysis of the phenotypic and genotypic characteristics plague pathogen strains from 2.MED4 2.MED1 phylogroups, representing main subspecies medieval Yersinia pestis biovar found in foci Caspian Sea region, which were epidemically active 20th century. evaluated biochemical properties, nutritional requirements, plasmid profiles Y. within phylogroups biovar. d...

One hundred twenty CTX-M-15-producing Escherichia coli strains isolated in 10 different hospitals from Paris (France), in the Hospital Charles Nicolle in Tunis (Tunisia), and in the Pasteur Institute in Bangui, Central African Republic (CAR), between 2000 and 2004 were studied. Eighty isolates, recovered from the three countries, were clonally related by repetitive extragenic palindromic PCR an...

One hundred twenty CTX-M-15-producing Escherichia coli strains isolated in 10 different hospitals from Paris (France), in the Hospital Charles Nicolle in Tunis (Tunisia), and in the Pasteur Institute in Bangui, Central African Republic (CAR), between 2000 and 2004 were studied. Eighty isolates, recovered from the three countries, were clonally related by repetitive extragenic palindromic PCR an...

The aim of this study was to characterize 49 Salmonella Enteritidis (SE) isolates from different sources (poultry, human, cow, poultry house environment) in Iran with respect to drug resistance, plasmid profile, and random amplified polymorphic DNA (RAPD) analysis. Antimicrobial susceptibility test to 29 agents found 33 resistance patterns among the isolates. No resistance was observed to danof...