PURPOSE To evaluate corneal biomechanical properties in eyes that had previously undergone penetrating keratoplasty (PK) using the ocular response analyzer (ORA). METHODS We recruited 26 patients who had received unilateral PK. Corneal hysteresis (CH), corneal resistance factor (CRF), Goldmann-correlated intraocular pressure (IOPg), and cornea-compensated intraocular pressure (IOPcc) were mea...

We cloned and sequenced the cDNAs for R-type pyruvate kinase (R-PK) from three homozygous PK patients. A point mutation (1151ACG to ATG) identified in the R-PK cDNA of PK Nagasaki was identical with that previously identified in an unrelated family, PK Tokyo. The mutation has also been identified in the PK L gene of a Lebanese family, PK Beirut, by Neubauer et al (Blood 77:1871, 1991). These re...

OBJECTIVES To determine and identify, unequivocally, if plasma kallikrein (PK) is present in the synovial fluid of patients with rheumatoid (RA), psoriatic (PA) and osteo (OA) arthritis, and to consider its functional importance in the inflamed joint. METHODS Therapeutically aspirated synovial fluids (pooled and individual samples, n = 66) were obtained from patients with arthritis. In additi...

t w t r i p s a t p s b f c ransferase, EC 2.7.1.40), a rate-controlling glycolytic nzyme, catalyses the transfer of a phosphoryl group rom phosphoenolpyruvate to ADP, yielding ATP and yruvate. PK exists in the cytosol and is functional as tetramer. Mammalian PK exists as four isoenzymes, hich are referred to as the L-, R-, M1-, and M2-types, espectively (1). The L-, R-, and M1-PK isoenzymes ar...

Plasma knife (PK) assisted UPPP is a modification of the procedure original described by Fujita for snoring. Plasma knife is used for tonsillectomy, hemostasis and to reduce scar contracture lowering incidence of dehiscence (1%) as a result of the reduced thermal injury. Cold knife resection of uvula is limited to the anterior mucosa and any submucosal; preserving the muscularis uvulae and post...

Repair of DNA damage is critical for genomic stability, and DNA-dependent protein kinase (DNA-PK) has an important role in repairing double-strand breaks. We examined whether the DNA-PK activity of peripheral blood lymphocytes (PBLs) was related to biochemical (prostate-specific antigen: PSA) relapse and radiation toxicity in prostate cancer patients who have received radiotherapy. A total of 6...

and Applied Analysis 3 2. Preliminary Results on Closed and Bounded Sequences of Linear Operators The subsequent result relies on the convergence of sequences of operators to limits so that we give the following simple result. Theorem 2.1. P∗1 / P∗2 ⇒ S P∗1 / S P∗2 ; for all P∗1, P∗2 ∈ L X ∩ clFP . Proof. Pk ∈ S P∗1 ⇔ Pk → P∗1 as k → ∞ in the strong operator topology. Thus, for any ε1 > 0, ∃n1 ...

We identified four distinct point mutations in homozygous pyruvate kinase (PK) variants in Japanese and Chinese patients with chronic nonspherocytic hemolytic anemia. All gene abnormalities were missense mutations that caused single amino acid substitutions. 1261A (Q421K) and 1436A (R436H), which were identified in PK Sendai and PK Shinshu, had been found in unrelated Japanese and Amish PK vari...

The glycolytic enzyme, L-pyruvate kinase (L-PK), plays an important role in hepatic glucose metabolism. Insulin and glucose induce L-PK gene expression, while glucagon and polyunsaturated fatty acids (PUFA) inhibit L-PK gene expression. We have been interested in defining the PUFA regulation of L-PK. The cis-regulatory target for PUFA action includes an imperfect direct repeat (DR1) that binds ...

BACKGROUND/AIMS M2 pyruvate kinase (M2-PK) is an enzyme that is produced in undifferentiated and proliferating tissues. This study aims to evaluate the usefulness of the immunochromatographic M2 pyruvate kinase (iM2-PK) for the screening of colorectal cancer (CRC) and premalignant lesions. METHODS Healthy volunteers and patients with colorectal neoplasia were enrolled in six academic hospital...