A Review of Optimization Strategies and the Advantages Disadvantages using Polymerase Chain Reaction with Confronting Two-Pair primers (PCR-CTPP) in Genomics Studies

during 2007 – 2008 different fruit gardens in shiraz (fars province, iran) were surveyed for samples being collected from infected apple, pear and quince trees. symptoms of the infected trees included water soaking of infected tissue, wilt and tissue necrosis, scorched and blackend of blossom bracts or young shoots. a total of 47 strains were isolated from infected trees using na medium. on the...

Xanthomonas campestris pv. (Xcc) is a harmful pathogen of the Brassicaceae agricultural plants in Serbian agroecological conditions. The bacterium survives soil, plant residues, seeds, and wild weedy plants. This study aimed to characterize Xcc isolates that persist field soil roots where they represent risk causing disease Collection samples was carried out period 2015 - 2017 four locations re...

PREMISE OF THE STUDY PCR amplification of the matK barcoding region is often difficult when dealing with multiple angiosperm families. We developed a primer cocktail to amplify this region efficiently across angiosperm diversity. METHODS AND RESULTS We developed 14 matK primers (seven forward, seven reverse) for multiplex PCR, using sequences available in GenBank for 178 taxa belonging to 123...