Snake venoms comprise a highly complex mixture of proteins, and there is also high interspecific intraspecific variability in their composition, even the same region. Our aim was to compare composition Bothrocophias myersi, Crotalus durissus Bothrops asper, snakes from Andean region Colombia by Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC). The were given research group under a...

Background: Sulfur mustard as a chemical warfare agent causes short and long-term pulmonary complications in its victims. MicroRNAs are known to act remarkable regulators of biological pathways, monitoring, treatment diseases including respiratory problems. In this study, we investigated the expression miR-106a-5p miR-106b-5p, two TGF-β signaling, well their target molecule, TGFβ1I1, peripheral...

Background and Objective: Blastocystis sp. is an anaerobic, single-celled intestinal parasite with global prevalence that is found in the lower digestive tract of humans and many animals, such as numerous types of vertebrates. To date, 17 genotypes have been identified. Nine of these genotypes are common in humans. Organism is found in both healthy and compromised individuals, hence its pathoge...

ABSTRACT: Present study aimed for detection, purification, quantification of Sind Krait (Bungarus sindanus) antivenom from chicken eggs and to determine extracted efficacy in mice. Hens’ three groups were immunized by sub-lethal doses venom with adjuvant paraffin oil+lecithin. Booster injected subcutaneously on pectorals muscles at multiple sites after every two weeks upto eight weeks. Antibodi...

Abstract Meningiomas are the most common primary tumors of central nervous system (CNS). Atypical meningioma (AM) recurs in 40% patients despite total resection and radiotherapy (RT). No consensus on optimal adjuvant management was found, it is difficult to identify insensitive RT real-life clinical practice. A promising group biomarkers represent microRNAs (miRNAs), short non-coding RNAs that ...

Gene expression quantification methods are important tools in the understanding of the molecular events underlying human diseases and in the identification of diagnostic and therapeutic targets. Generally, the messenger RNA (mRNA) used for these analyses is derived from human biopsies obtained after surgery. As a consequence, several steps during tissue handling have to be carefully controlled ...

Isolation of high-quality RNA is one of the most crucial methods in molecular biology. RNA extraction from woody plants has been problematic due to the presence of rigid and woody tissues, large amounts of polysaccharides, polyphenols and other secondary metabolites. Here we present a suitable protocol for RNA isolation from a wide range of woody plants that includes eight gymnosperms and four ...

An efficient DNA isolation protocol specifically modified to get pure quality DNA required for molecular studieshas been reported in this paper. Some aquatic plants (Potamogeton spp., Ceratophyllum demersum and Myriophyllum spicatum) were used for the study. The protocol developed will be useful in getting high and pure DNA. Instead of using the available DNA extraction kits, this protocol can ...