The polarity of kinetochore microtubules (MTs) has been studied in lysed PtK1 cells by polymerizing hook-shapdd sheets of neurotubulin onto the walls of preexisting cellular MTs in a fashion that reveals their structural polarity . Three different approaches are presented here : (a) we have screened the polarity of all MTs in a given spindle cross section taken from the region between the kinet...

In the present research, vibrational behavior of anisotropic protein microtubules (MTs) immersed in cytosol via Cooper–Naghdi shell model is investigated. MTs are hollow cylindrical structures in the eukaryotic cytoskeleton which surrounded by filament network. The temperature effect on vibration frequency is also taken into account by assuming temperature-dependent material properties for MTs....

Tannic acid fixation reveals differences in the number of protofilaments between microtubules (MTs) in the nematode Caenorhabditis elegans. Most cells have MTs with 11 protofilaments but the six touch receptor neurons (the microtubule cells) have MTs with 15 protofilaments. No 13-protofilament (13-p) MT has been seen. The modified cilia of sensory neurons also possess unusual structures. The ci...

This study was conducted to determine caeca micro flora and tannin effects on amino acid availability (AAA) values for different grain sorghum cultivars (GSC). Three GSCs including low tannin (0.09%, LTS), medium tannin (0.19%, MTS) and high tannin (0.37%, HTS) were selected to determine AA availability compare to the corn by using the true metabolizable energy (TME) assay with both intact (INT...

The host cell microfilaments and microtubules (MTs) are known to play a critical role in the life cycles of several pathogenic intracellular microbes by providing for successful invasion and promoting movement of the pathogen once inside the host cell cytoplasm. Orientia tsutsugamushi, an obligate intracellular bacterium, enters host cells by induced phagocytosis, escapes to the cytosol, and th...

Metallothioneins levels (MTs) in the clams Callista chione and Venus verrucosa, collected from two coastal sites in Greece, were determined and quantified by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and a spectrophotometric assay (Ellman's reaction). SDS-PAGE separation in the digestive gland, which represents the hepato-pancreas in clams, demonstrated the presence of MTs similar to ma...

The polarity of kinetochore microtubules (MTs) has been studied in lysed PtK1 cells by polymerizing hook-shaped sheets of neurotubulin onto walls of preexisting cellular MTs in a fashion that reveals their structural polarity. Three different approaches are presented here: (a) we have screened the polarity of all MTs in a given spindle cross section taken from the region between the kinetochore...

BACKGROUND Kaempferol, a natural flavonoid, has been shown to induce cancer cell apoptosis and cell growth inhibition in several tumors. Previously we have conducted a full investigation on the chemical constituents of Gynura medica, kaempferol and its glycosides are the major constituents of G. medica. Here we investigated the growth inhibition and apoptosis induction effect of kaempferol extr...