This paper reports a high-throughput n × m well array PCR chip with an accurate and reliable method for sample loading and primers mixing. PCR mixture could be precisely introduced into each reaction chamber, which was prespotted and dried up with each primer set, by sucking the air through PDMS gas permeable wall. In this approach, the mixing of primer and sample at constant ratio was achieved...

The fluidic oscillator is a device that generates an oscillating jet when supplied with fluid at pressure. The oscillator has no moving parts – the creation of the unsteady jet is based solely on fluid-dynamic interactions. Fluidic oscillators can operate at frequencies ranging up to 20 kHz, and are useful for flow control applications. The fluidic oscillator evaluated in the current study is c...

BACKGROUND Lung volume measurement by nitrogen washout is widely used in infants, though a lack of accuracy and changes of calibration over time have been reported. The potential sources of error were explored in order to increase the accuracy and reliability of the technique. METHODS A commercial system for nitrogen washout and a 0.5 litre calibrating syringe as a lung model were used to per...

Conventional flow injection systems for aquatic environmental analysis typically comprise large laboratory benchscale equipment, which place considerable constraints for portable field use. Here, we demonstrate the use of an integrated acoustically driven microfluidic mixing scheme to enhance detection of a chemiluminescent species tris(2,2'-bipyridyl)dichlororuthenium(II) hexahydrate-a common ...

We propose a new way of implementing a biomolecular computer in the laboratory, using deoxyribozyme logic gates inside a microfluidic reaction chamber. We build upon our previous work, which simulated the operation of a deoxyribozymebased flip-flop and oscillator in a continuous stirred-tank reactor (CSTR); unfortunately, using these logic gates in a laboratory-size CSTR is too expensive becaus...

UNLABELLED We have introduced a novel, parallel design into a new clinical bymixer (patent pending), named for the bypass of a constant fraction of total flow through a mixing chamber. Over a wide range of tidal volumes (300-1200 mL), frequency (6-20 breaths/min), and PCO(2) (6-50 mm Hg), the bymixer provided accurate measurement of mixed expired gas fractions in the ventilation circuit compare...

Interaction between small-scale turbulence and cloud particles is a key issue in warm rain formation mechanism, which is an important meteorological and climatological challenge [1],[2]. We investigate this interaction observing motion of cloud droplets in a glass walled chamber 1m deep, 1m wide and 1.8m high [3]. The second smaller chamber, placed above the main one is filled with water drople...

Pre-chamber spark-ignition (PCSI), either fueled or non-fueled, is a leading concept with the potential to enable diesel-like efficiency in medium-duty (MD) and heavy-duty (HD) natural gas (NG) engines. However, inadequate scientific base simulation tools describe/predict underlying processes governing PCSI systems one of key barriers market penetration for MD/HD NG To this end, experiments wer...

A Kelvinox 400 dilution refrigerator with the ability to load samples onto the mixing chamber from the bottom of the cryostat has been combined with an ultrahigh-vacuum (UHV) deposition chamber equipped with molecular beam sources. The liquid helium cooled sample transfer mechanism is used in a manner that allows films to be grown on substrates which are kept at temperatures of order 8K with ch...