Prognosticators evaluating survival in breast cancer vary in significance in respect to lymph node status. Studies have shown e.g. that HER2/neu immunohistochemistry or HER2/neu gene amplification analysis do perform well as prognosticators in lymph node positive (LN +) patients but are less valuable in lymph node negative (LN -) patients. We collected data from different studies and tried to e...

Protein synthesis and RNA synthesis during mitosis were studied by autoradiography on mammalian tissue culture cells. Protein synthesis was followed by incubating hamster epithelial and human amnion cells for 10 or 15 minutes with phenylalanine-C(14). To study RNA synthesis the hamster cells were incubated for 10 minutes with uridine-C(14). Comparisons of the synthetic capacity of the interphas...

Synchronized cells of the cellular slime mould Dictyostelium discoideum were prepared by mitotic wash-off. Cell counts and DNA synthesis measurements indicated a high degree of synchrony. Cells from each phase of the cell cycle were fluorescently labelled and mixed with unlabelled asynchronous cells. Cells that were in S-phase and very early G2 at the onset of starvation demonstrated a strong t...

Gastrointestinal stromal tumors have a wide spectrum of biologic behavior, and occasional cases show liver metastases. The modified risk grade based on tumor size and mitotic counts has been proposed to predict the biologic behavior in gastric gastrointestinal stromal tumors. Blood vessel invasion (BVI) is important in the development of metastasis of various kinds of cancer. The aim of this st...

The hemocyte types, in addition to total and differential hemocyte counts were studied in parasitized and unparasitized Anastrepha obliqua larvae at the beginning and at the end of the third instar. In both developmental phases, in parasitized and unparasitized larvae, prohemocytes, plasmatocytes, granulocytes, adipohemocytes, spherulocytes and oenocytoids cells were observed. Mitotic figures i...

were analyzed in mice. Cell counts in unit volumes of bone marrow, mitotic indices, and relative frequencies of individual elements were estimated 1, 3, 6, and 24 hr and 3, 6, and 10 days after drug administration. It was found that changes in mitotic index occurred prior to changes in cell count. Mitotic index and the cell count decreased from Hr 1 reaching minimum values after 24 and 72 hr, r...

In vivo studies of Ehrlich ascites tumor cells during the first 5 days of growth in peritoneal cavities of mice consisted of the following: 1. Determination of growth curves by direct enumeration of cells. 2. Estimation of the duration of each phase of the mitotic cycle based on incidence of cells in different phases. 3. Radioautographic studies to determine the proportion of cells in different...

tissue mitotic counts, which also peaked at diestrus 1. Radioactivities in the acid-soluble fraction of the incubated tissues were not significantly different for either labeled precursors throughout the estrous cycle. Autoradiographic studies of tissues collected after incubation with [3H]thymidine showed that silver grains were localized over cell nuclei. The data indicate that the growth of ...

tissue mitotic counts, which also peaked at diestrus 1. Radioactivities in the acid-soluble fraction of the incubated tissues were not significantly different for either labeled precursors throughout the estrous cycle. Autoradiographic studies of tissues collected after incubation with [3H]thymidine showed that silver grains were localized over cell nuclei. The data indicate that the growth of ...

Background: Gastrointestinal stromal tumors (GISTs) constitute the majority of gastrointestinal mesenchymal tumors. They usually express a proto-oncogen protein called CD117 detected by immunohistochemistry. This study investigated the differentiation of GISTs as well as the risk of aggressive behaviors in GISTs from surgically-treated patients in university affiliated hospitals. Methods: The c...