Non-invasive assessment of the perfusion capacity of tissue engineered constructs grown on the chorioallantoic membrane by MRI is often hampered by motion artifacts. Therefore, we examined the suitability of three anesthetic regimes for sufficient sedation of the chick embryo. Medetomidine at a dosage of 0.3 mg/kg, was compared to thiopental at 100 mg/kg and ketamine/midazolam at 50 mg/kg and 1...

The goal of this study was to establish an easy-to-perform, reliable, and safe protocol for intraperitoneal injection anaesthesia in rats, as well as an easy method to assess anaesthesia depth under routine clinical conditions. Seventy Wistar rats (35 males and 35 females) were used to evaluate intraperitoneal anaesthesia with ketamine (75 mg/kg) combined with one of the following central nervo...

This paper presents a novel assay that allows a quick and robust assessment of the effects of biocides on the initial settling and establishment of marine photoautotrophic biofilms including the multitude of indigenous fouling organisms. Briefly, biofilms are established in the field, sampled, comminuted and re-settled on clean surfaces, after 72h chlorophyll a is measured as an integrating end...

Resting-state functional magnetic resonance imaging (rsfMRI) is a translational imaging method with great potential in several neurobiologic applications. Most preclinical rsfMRI studies are performed in anesthetized animals, but the confounding effects of anesthesia on the measured functional connectivity (FC) are poorly understood. Therefore, we measured FC under six commonly used anesthesia ...

experimental determination of solubility and ternary phase diagram of chiral compound are of tedious and time consuming tasks, and in many cases, there is not enough experimental data for different enantiomeric compositions to access the experimental ternary phase diagram. using thermodynamic models with predictive capability, having less dependency on experimental data, affords a great advanta...

This study was performed to distinguish central and peripheral a -adrenoceptors in the inhibition of rat pineal melatonin synthesis. The 2 rats received lipoor hydrophilic a -adrenoceptor ligand injections at middark; after 1 or 2 h the pineal melatonin contents were 2 measured. The lipophilic agonist medetomidine (100 mg/kg s.c.) suppressed the melatonin contents significantly, while the hydro...

The quality, duration and cardiorespiratory effects of 3 anaesthetic drugs were determined in 15 healthy adult male dogs. Dogs were divided into 3 equal groups, each group was intravenously administered a combination of 2 of the following drugs: midazolam (0.3 mg/kg), medetomidine (20 μg/kg) and ketamine (10 mg/kg). Induction, anaesthetic and recovery times were recorded. Heart rate, systolic a...