نتایج جستجو برای: lipase gene

تعداد نتایج: 1154330  

Journal: :Biochemical Society transactions 2000
J Thompson C Taylor T W Wang

A cDNA clone encoding a lipase that is up-regulated in senescing leaves and flower petals has been isolated by screening an expression library. The abundance of the lipase mRNA increases as flowers and leaves begin to senesce, and expression of the gene is also induced by treatment with ethylene. Transgenic Arabidopsis plants in which levels of the senescence-induced lipase protein have been re...

Journal: :Journal of microbiology and biotechnology 2013
Arild Ranlym Arifin Soon-Ja Kim Joung Han Yim Antonius Suwanto Hyung Kwoun Kim

Lipase-producing bacterial strains were isolated from Antarctic soil samples using the tricaprylin agar plate method. Seven strains with relatively strong lipase activities were selected. All of them turned out to be Bacillus pumilus strains by the 16S rRNA gene sequence analysis. Their corresponding lipase genes were cloned, sequenced, and compared. Finally, three different Bacillus pumilus li...

Journal: :Journal of bacteriology 1995
R G Kok J J van Thor I M Nugteren-Roodzant B Vosman K J Hellingwerf

Acinetobacter calcoaceticus BD413 produces an extracellular lipase, which is encoded by the lipA gene. Five lipase-deficient mutants have been generated via random insertion mutagenesis. Phenotypic characterization of these mutants revealed the presence of as many as four lipolytic enzymes in A. calcoaceticus. Biochemical evidence classified four of the mutants as export mutants, which presumab...

Journal: :Nucleic acids research 1985
F Götz F Popp E Korn K H Schleifer

The lipase gene from Staphylococcus hyicus subsp. hyicus was cloned in Staphylococcus carnosus and Escherichia coli. In both host organisms the lipase gene is expressed and the enzyme is released to the medium. The cloned DNA insert is 2.5 kb in length and DNA sequencing has revealed the location of the gene, the ribosomal binding site and the presence of a typical signal sequence. The open rea...

Journal: :Journal of advances in microbiology 2022

Lipase is a glycerol ester hydrolase that hydrolyzes esters of triglycerides. This study aimed to isolate, screen and characterize lipolytic fungi with lipA gene from soil environments. Fungi [molds yeasts] were isolated contaminated samples randomly collected Palm oil mills, Abattoirs Automobile servicing workshops in Eleme Obio Akpor local governments Rivers State, Nigeria using standard micr...

Journal: :The protein journal 2014
Xiaoli Tan Suzhen Yan Renke Tan Zhiyan Zhang Zheng Wang Jie Chen

The GDSL esterase and lipase families play important roles in abiotic stress, pathogen defense, seed development and lipid metabolism. Identifying the lipase activity of the putative GDSL lipase is the prerequisite for dissecting its function. According to the sequence similarity and the conserved domains, we cloned the Brassica napus BnGLIP gene, which encodes a GDSL-like protein. We failed to...

Journal: :Journal of virology 2003
Kangayam M Ponnuvel Hiroshi Nakazawa Seiichi Furukawa Ai Asaoka Jun Ishibashi Hiromitsu Tanaka Minoru Yamakawa

A protein showing strong antiviral activity against Bombyx mori nucleopolyhedrovirus (BmNPV) was purified from the digestive juice of B. mori larvae. A homology search of the deduced amino acid sequence of the protein cDNA revealed 56% homology with Drosophila melanogaster lipase and 21% homology with human lipase. As lipase activity of the protein was confirmed in vitro, this protein was desig...

2011
E Mobarak-Qamsari R Kasra-Kermanshahi Z Moosavi-nejad

BACKGROUND AND OBJECTIVES Lipases are particularly important due to the fact that they specifically hydrolyze acyl glycerol, oils and greases, which is of great interest for different industrial applications. MATERIALS AND METHODS In this study, several lipase-producing bacteria were isolated from wastewater of an oil processing plant. The strain possessing the highest lipase activity was ide...

1996
Karl-Erich Jaeger Frank Rosenau Dietmar Lang Albin Zonta Manfred T. Reetz

Lipase genes originating from the Gram-negative bacteria Serrutiu marcescens and Pseudomonus urruginosa were cloned. S. marcescens lipase was overexpressed in Escherichia coli yielding inclusion bodies which were purified and finally refolded to give enzymatically active lipase. The lipase operon of P. aeruginosa consisting of genes 1ipA and lip14 was cloned behind the T7 410 promoter and overe...

Journal: :The Journal of biological chemistry 1989
T G Kirchgessner R C LeBoeuf C A Langner S Zollman C H Chang B A Taylor M C Schotz J I Gordon A J Lusis

We report here a study of the developmental and genetic control of tissue-specific expression of lipoprotein lipase, the enzyme responsible for hydrolysis of triglycerides in chylomicrons and very low density lipoproteins. Lipoprotein lipase (LPL) mRNA is present in a wide variety of adult rat and mouse tissues examined, albeit at very different levels. A remarkable increase in the levels of LP...

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