Callus induction responses and regeneration through callus-mediated SE were studied from evening primrose (Oenothera biennis L.) as an important medicinal plant of Onagraceae mainly known for its gamma-linoleic acid (GLA) content. The effects of cytokinins, 6-Benzylaminopurine (BAP) and N-(2-furfurylamino)1-H-purine-6-amine [Kinetin (Kin)] and their concentrations (0, 0.5, 1.0 and 1.5 mg/l) in ...

Many plant organs have the ability to regenerate a new plant after detachment or wounding via de novo organogenesis. During de novo root organogenesis from Arabidopsis thaliana leaf explants, endogenic auxin is essential for the fate transition of regeneration-competent cells to become root founder cells via activation of WUSCHEL-RELATED HOMEOBOX 11 (WOX11). However, the molecular events from l...

The present investigation was carried out to study the In vitro shoot multiplication and callogenesis in Gladiolus. For this purpose, cormel, meristem and leaf explants were chosen. Different concentrations of BAP i. e., 0.5, 1.0, 1.5, 2.0 or 2.5 mgL (2.22, 4.44, 6.66, 8.88 or 11.10 μM) were supplemented to MS medium. Both cormel and meristem explants showed maximum multiplication on MS medium ...

BACKGROUND AND AIMS Understanding the fate and dynamics of cells during callus formation is essential to understanding totipotency and the mechanisms of somatic embryogenesis. Here, the fate of leaf explant cells during the development of embryogenic callus was investigated in the model legume Medicago truncatula. METHODS Callus development was examined from cultured leaf explants of the high...

Cumin (Cuminum cyminum L.) as a member of the Apiaceae family is one of the most important medicinal plants in Iran. The purpose of this study is to evaluate the effect of plant growth regulators and explant type on callus induction in cumin. For this purpose the cumin seeds (Kuhbanan accession) were disinfected with sodium hypochlorite and alcohol and cultured on MS basal medium. Leaf and hypo...

the present investigation was conducted to study the effects of type and concentration of auxin and cytokinin on callus induction of three strawberry (kurdistan, parose and camarosa) cultivars. for this purpose, leaf blade, nodal, petiole, receptacle, stamen and flower bud explants were cultured on murashige and skoog (ms) medium at three separate experiments included 2, 4 dichlorophenoxyacetic...

Leaf and stem explants excised from young plant of B.diffusa  were cultured on Murashige and Skoog (MS) medium containing agar (0.8%), sucrose (2.5%) and varied concentration of Indole butyric acid (IBA), Napthyl acetic acid (NAA), 2,4 - dichloro phenoxy acetic acid (2,4-D) and Picrolam. Leaf explants has given better response for both rooting and callus formation. IBA in a concentration of 5μM...

An efficient plant regeneration protocol has been developed from leaf explants of Stevia rebaudiana, an economically and medicinally important herbaceous plant species belonging to the family Asteraceae. Plant regeneration and multiple shoot proliferation was initiated from leaf explants cultured on MS medium supplemented with various concentrations of 6-benzyladenine (BA) and kinetin (KN) in c...

An efficient plant regeneration protocol was developed from leaf and internode (stem) explants of Bacopa monniera L. on MS basal medium, supplemented with Cytokinins like BAP, KN, and TDZ used singly. The leaf cultures expressed maximum plant regeneration potential with 138.9 ± 0.50 shoots per explants on MS + 2.0 mg/ l BAP. The in vitro regenerated dwarf shoots were further elongated on MS med...