We used an RIA and inhibition of enzyme activity to monitor the changes in mass and catalytic concentrations of the aspartate aminotransferase (EC 2.6.1.1;AST) isoenzymes in serum after myocardial infarction. Cytosolic (c-AST) and mitochondrial (m-AST) forms of AST were present in sera from all 38 of our patients. Although the immunological and catalytic concentrations of both isoenzymes correl...

Metastable creatine kinase MM isoenzyme was isolated and partially purified from homogenates of myocardium and skeletal muscle by gradient elution on carboxymethyl cellulose. This variant isoenzyme migrated between the MM and MB isoenzymes on agarose electrophoresis, accounted for 3.5% of the total creatine kinase activity in each tissue, was not a macromolecule, and had stable electrophoretic ...

Activity of serum/plasma beta-N-acetylhexosaminidase (EC 3.2.1.52) was determined by means of a continuous spectrophotometric method using 3,3'-dichlorophenylsulphonphthaleinyl-N-acetyl-beta-D-glucosaminid e as substrate, with very satisfactory results. Incubation of an undiluted aliquot (1 ml) of samples at 52 degrees C for 8 hours with an adjusted pH 5.5-6.0 provoked only the inactivation of ...

We examined the effect of storing human plasma or extracts of prostate at -90 degrees C on the activity of creatine kinase and lactate dehydrogenase and isoenzyme distribution. Enzyme activities were unaltered during storage for as long as six weeks. If these preparations were thawed only once at 2 to 4 degrees C, they could be stored for as long as 165 days at -90 degrees C with no change in i...

2. Dawson DM, Fine IH. Creatine kinase in human tissue. Arch Neurol 1966;16:175-80. 3. Van Der Veen KJ, Willebrands AF. Isoenzyme of creatune phosphokunasein tissue extract and in normal and pathologic sera. Clin Chim Acts 1966;13:312-5. 4. Wagner GS, Roe CR, Liinbird LF, et al. The importanceof identificationof myocardiac specific isoenzyme of creatune phosphokinase (MB form) in the diagnosis ...

An enzymatic, immunochemical test system has been developed which allows quantitation of minute amounts of the Regan isoenzyme of alkaline phosphatase. The basis of the method is the concentration (tenfold) of the Regan isoenzyme by reaction with a monospecific antiserum to placenta! alkaline phosphatase, insolubilized by polymerization with ethyl chloroformate. This concentration is achieved b...

Isoenzyme and random amplified polymorphic DNA (RAPD) markers were used to characterize the genetics of geographic variation among population samples of Ascaris suum from midwestern localities. Independent estimates of fixation indices (FST) based on isoenzyme and RAPD markers showed the same general patterns of differentiation and substantial statistical correlation (r = 0.70). Of the total es...

Serum adenylate kinase (AK) activity was measured in cerebral vascular diseases and the AK isoenzyme pattern was studied in the patients suffering from cerebral infarction, cerebral hemorrhage, myocardial infarction, and polymyositis. There was a significant difference in AK activity between the control subjects and patients with acute cerebral infarction. Serum AK activity showed a peak on the...

The structural relationships among human alkaline phosphatase isoenzymes from placenta, bone, kidney, liver and intestine were investigated by using three criteria. 1. Immunochemical characterization by using monospecific antisera prepared against either the placental isoenzyme or the liver isoenzyme distinguishes two antigenic groups: bone, kidney and liver isoenzymes cross-react with anti-(li...

Histological examination of supraclavicular lymph node tissue obtained at biopsy from a 63-year-old man disclosed metastatic small-cell carcinoma. On admission and for four days subsequently, total lactate dehydrogenase (LD; EC 1.1.1.27) activity in serum was 6.5 times normal; studies of LD isoenzyme showed persistently increased LD-1, with LD-1 greater than LD-2. Isoenzyme electrophoresis of t...