UV irradiation of quiescent human fibroblasts immediately triggers the appearance of the nuclear protein cyclin/proliferating cell nuclear antigen (PCNA) as detected by indirect immunofluorescent staining after methanol fixation. This was found to be independent of new synthesis of cyclin/PCNA by two-dimensional gel analysis and cycloheximide treatment. The intensity of the immunofluorescent st...

The progress of antisense DNA therapy demands development of reliable and convenient methods for sequencing short single-stranded oligonucleotides. A method of phosphorothioate antisense DNA sequencing analysis using UV detection coupled to capillary electrophoresis (CE) has been developed based on a modified chain termination sequencing method. The proposed method reduces the sequencing cost s...

Procedures for the generation and collection of transient UV-visible absorbance data are briefly reviewed. Problems associated with signal generation (scattered light, inhomogeneous distribution of transients, instability of pulsed light sources), signal detection (averaging, filtering), and signal analysis in kinetic and spectrographic flash photolysis are addressed. Methodology for the fittin...

We present a joint theoretical and experimental investigation of the optoelectronic properties of CuVO3, CuNbO3 and Cu5Ta11O30 materials for potential photocatalytic and solar cell applications. In addition to the experimental results obtained by powder X-ray diffraction and UV-Vis spectroscopy of the materials synthesized under flowing N2 gas at atmospheric pressure via solid-state reactions, ...

A novel indirect electrochemical protocol for the electroanalytical detection of phenols is presented for the first time. This methodology is demonstrated with the indirect determination of the target analytes phenol, 2-chlorophenol, 4-chlorophenol and 2,4-dichlorophenol through an electrochemically adapted optical protocol. This electrochemical adaptation allows the determination of the above ...

We consider electrically neutral complex vector particles $V$ below the GeV mass scale that, from a low energy perspective, couple to photon via higher dimensional form factor interactions. derive ensuing astrophysical constraints by considering anomalous loss Sun, Horizontal Branch, and Red Giant stars as well SN1987A that arise pair-production in these environments. Under assumption dark stat...

We study potential signals of neutralino dark matter indirect detection by neutrino telescopes in a wide range of CMSSM parameters. We also compare with direct detection potential signals taking into account in both cases present and future experiment sensitivities. Only models with neutralino annihilation into gauge bosons can satisfy cosmological constraints and current neutrino indirect dete...

Deep UV fluorescence detection at 266-nm excitation wavelength has been realized for sensitive detection in microchip electrophoresis. For this purpose, an epifluorescence setup was developed enabling the coupling of a deep UV laser into a commercial fluorescence microscope. Deep UV laser excitation utilizing a frequency quadrupled pulsed laser operating at 266 nm shows an impressive performanc...

A rapid, simple and sensitive high-performance liquid chromatography method was developed for determination of ciprofloxacin in plasma by means of ultraviolet detection. Ofloxacin was used as an internal standard and separation carried on a Novapak C18 column using a mobile phase of 0.01 M phosphate buffer (pH =2.6): methanol (82: 18 v/v). Extraction of drug was performed from plasma by liquid-...

Semiconductor-based ultraviolet (UV) photodiodes have been continuously developed that can be widely used in various commercial, civilian areas, and military applications, such as optical communications, missile launching detection, flame detection, UV radiation calibra‐ tion and monitoring, chemical and biological analysis, optical communications, and astro‐ nomical studies, etc. [1-2]. All th...