high-performance liquid chromatography (hplc) methods employing ultraviolet (uv) detector are not sufficiently sensitive to measure the low plasma concentrations following single oral dose of imipramine. therefore, in the present study a simple, rapid and yet sensitive hplc method with uv detection was developed and validated for quantitation of imipramine in human plasma samples. an efficient ...

Introduction: Boron possesses widespread properties and is important for human and animal nutrition. Since Boron is rapidly bioavailable, the objective of the present study was to determine whether acute (hourly or daily), and weekly supplementationcould have any significant biological effects on the synthesis of steroids as well as inflammatory biomarkers. Methods: Eight male volunteers part...

In current clinical practice, plasma and blood glucose are used interchangeably with a consequent risk of clinical misinterpretation. In human blood, glucose, like water, is distributed between erythrocytes and plasma. The molality of glucose (amount of glucose per unit of water mass) is the same throughout the sample, but the concentration is higher in plasma because the concentration of water...

Objectives of this study were to adapt a commercial human protein C (PC) colorimetric assay for use in dogs and to investigate effects of various storage conditions. The human assay was modified by using pooled canine plasma for calibration and by increasing the activation time. PC activity was measured in fresh canine plasma and in plasma stored under various conditions. PC activity of some st...

We describe a fluorometric procedure, with use of fluorescamine, for determining plasma amino nitrogen in a 10-mul sample of deproteinized plasma. Values for apparently healthy human subjects agree well with those obtained by spectrophotometry. The simplicity and speed of the assay recommends it as a screening procedure for detecting aminoacidopathies.

Objective: There exists a variety of methodologies for the quantitation of effective renal plasma flow (ERPF) with 99"'Tc-MAG3, from very accurate in vitro techniques to less accurate, yet reliable and easy gamma camera methods. This study compares a new technique that uses a single blood sample but does not require the preparation of a standard, with the in vitro single sample ERPF determinati...

the total vitamin c content in human plasma is widely accepted as an indicator of the tissue status of vitamin c. a liquid chromatography method with ultraviolet detector (264 nm) for measuring ascorbic acid in human plasma was developed. a c18 reversed-phase column and cetrimide as an ion-pairing agent was employed. ascorbic acid (aa) was measured after reducing l-dehydroascorbic acid to l-asc...

Circulating biomarkers are on the verge of becoming powerful diagnostic tools for various human diseases. However, the complex sample composition makes it difficult to detect biomarkers directly from blood at the bench or at the point-of-care. Blood cells are often a source of variability of the biomarker signal. While the interference of hemoglobin is a long known source of variability, the re...