A major protein-bound ligand in the serum of chronic hemodialysis patients was isolated from heat-deproteinized uremic serum by "high-performance" liquid chromatography (HPLC). The isolated compound was identified as 3-carboxy-4-methyl-5-propyl-2-furanpropionic acid by use of liquid secondary-ion mass spectrometry and gas chromatography-mass spectrometry. On HPLC, an authentic sample of this co...

The paper focuses on the analysis and detection of electroactive compounds using high-performance liquid chromatography (HPLC) combined with electrochemical detection (EC). The fabrication and utilization of electrochemically treated carbon fiber microelectrodes (CFMs) as highly sensitive amperometric detectors in HPLC are described. The applied pretreatment procedure is beneficial for analytic...

the lack of authentic standards has limited the quantitative analysis of herbal drugs in biological samples. the present work demonstrated a practicable strategy for the assay of herbs and their metabolites independent of authentic standards. a liquid chromatography–electrospray ionization–mass spectrometry (lc–esi–ms) method for the qualitative and quantitative determination of the metabolites...

objective(s) developing and validating a simple, efficient, reproducible and economic reversed phase high performance liquid chromatographic (rp-hplc) method for the quantitative determination of pefloxacin in bulk material, tablets and in human plasma. materials and methods a shim-pack clc-ods column and a mobile phase constituting acetonitrile: 0.025 m phosphoric acid solution (13:87 v/v, ph ...

the occurrence and fate of trace-level contamination of pharmaceuticals in the aquatic environment has been recognized as one of the emerging issues in environmental chemistry. the effluents of wastewater treatment plants, usually directly emitted to the environment, often contain the anti-inflammatory drug diclofenac. diclofenac was chosen because it is of high consumption; by background liter...

this paper describes two rapid, sensitive and specific methods for the determination of fulvestrant in pharmaceutical preparations by high performance liquid chromatography (hplc) and linear sweep voltammetry (lsv). hplc method was used to study the degradation behaviour. fulvestrant was subjected to degradation under the conditions of hydrolysis (acid and alkali), oxidation (30% h2o2). the lin...

During the past decade, technological innovations based on new knowledge of the principles underlying separations processes have transformed column liquid chromatography, the oldest chromatographic technique, into a sophisticated tool for rapidly achieving the most difficult of separations and analyses. Applications of this modern version called High Performance Liquid Chromatography (HPLC) are...

Abbreviation Key HBSS = Hanks balanced salt solution, HPLC = high performance liquid chromatography, IL-2 = interleukin-2, NK = natural killer, SAPLIP = saposin-like protein.

Pharmaceutical analysis based on chromatographic separation is an important part of studies aimed at developing routine quality analysis of drugs. High-performance liquid chromatography (HPLC) is one of the main analytical techniques recommended for drug analysis. Although it meets many criteria vital for analysis, it is time-consuming and uses a relatively high amount of organic solvents compa...