Epoxide hydrolase from Agrobacterium radiobacter AD1 catalyzes the enantioselective hydrolysis of styrene oxide with an E value of 16. The (R)-enantiomer of styrene oxide is first converted with a kcat of 3.8 s-1, and the conversion of the (S)-enantiomer is inhibited. The latter is subsequently hydrolyzed with a kcat of 10.5 s-1. The pre-steady-state kinetic parameters were determined for both ...