Abstract : Foot and Mouth Disease is one of  the important live stocks contagious viral disease caused by Aphtovirus genus ,  that is belong to family RNA virus  picornaviride. The important characteristic of FMD virus is high mutation that give rise to diversity of Antigen in surface of Neutralizing proteins. For this reason FMD virus have 7 distinct serotype and many subtype. Vaccination is o...

Background: Weissellicin 110 is the only bacteriocin reported in Weissella cibaria up to now. This bacteriocin represents several unique features. This is the first report on the gene sequence that encodes for the bacteriocin. Objectives: Providing a rapid detection method to isolate the weissellicin 110 encoding gene and determination of the bacteriocin distribution were the objectives. Materi...

Abstract While the cost and time for assembling a genome has drastically decreased, it still remains challenge to assemble highly contiguous genome. These challenges are rapidly being overcome by integration of long-read sequencing technologies. Here, we use improve contiguity threespine stickleback fish (Gasterosteus aculeatus) genome, prominent genetic model species. Using Pacific Biosciences...

Although many genomes are available for download today, the underlying technologies should not be taken for granted. By using shotgun sequencing techniques and a gauntlet of informatics, we are able to produce high-quality DNA sequence. We will first look at some of the robotics and chemistries of preparing DNA as samples for the sequencing instruments. Then we will look at the series of applic...

Next-generation sequencing technology has enabled genome-wide analysis of 5mC nucleotides at single-nucleotide resolution. Previous methods for whole-genome bisulfite sequencing (WGBS) yielded reduced genomic representation due to required DNA shearing, ligation of methylated sequencing adapters, and bisulfite conversion of unmethylated cytidine residues prior to sequencing. One disadvantage of...