Neisseria meningitidis causes invasive meningococcal disease in infants, toddlers, and adolescents worldwide. DNA sequence-based typing, including multilocus sequence typing, analysis of genetic determinants of antibiotic resistance, and sequence typing of vaccine antigens, has become the standard for molecular epidemiology of the organism. However, PCR of multiple targets and consecutive Sange...

1. Abstract 2. Introduction 3. Genotyping methods 3.1. Genetic markers 3.1.1. Insertion sequences 3.1.2. Short repetitive DNA sequences 3.2. Restriction fragment length polymorphism analysis 3.2.1. IS6110 RFLP analysis 3.2.2. PGRS RFLP analysis 3.3. PCR-based typing 3.3.1. Mixed-linker PCR 3.3.2. Spoligotyping 3.3.3. VNTR and MIRU-VNTR typing 3.4. Comparison of genotyping methods 4. Molecular e...

A total of 108 S. aureus isolates from 16 major hospitals located in 14 different provinces in China were characterized for the profiles of 18 staphylococcal enterotoxin (SE) genes, 3 exfoliatin genes (eta, etb and etd), and the toxic shock syndrome toxin gene (tsst) by PCR. The genomic diversity of each isolate was also evaluated by pulsed-field gel electrophoresis (PFGE), multilocus sequence ...

INTRODUCTION .......................................................................................................................................................149 TAXONOMY ...............................................................................................................................................................149 Historical Features ........................................

Multilocus sequence typing (MLST) based on the 16S RNA, pduF, glnA, and manB genes was developed for Salmonella, and its discriminatory ability was compared to those of pulsed-field gel electrophoresis (PFGE) and serotyping. PFGE differentiated several strains undifferentiable by serotyping, and 78 distinct PFGE types were identified among 231 Salmonella isolates grouped into 22 serotypes and 1...

A PCR method previously developed for typing Mycobacterium avium was used to characterize the genetic diversity of M. avium strains isolated from swine (n = 90) and humans (n = 24). The strains were identified with IS901 PCR and IS1245 PCR: 38 strains were of IS901+ and IS1245+ genotype (M. avium subsp. avium) and 76 strains were of IS901– and IS1245+ genotype (M. avium subsp. hominissuis). All...

The International HapMap Project and the arrival of technologies that type more than 100,000 SNPs in a single experiment have made genome-wide single nucleotide polymorphism (GW-SNP) assay a realistic endeavor. This has sparked considerable debate regarding the promise of GW-SNP typing to identify genetic association in disease. As has already been shown, this approach has the potential to loca...

Classical Swine Fever Virus (CSFV) is the cause of an economically important and contagious disease in all age groups of pigs. Advances in molecular methods have facilitated genetic typing of this virus which is useful for classification, to trace patterns of virus spread and exposing the weaknesses in control strategies. Moreover, the genetic comparison of the isolates obtained from a series o...

The present study aimed at the assessment of genetic relatedness among the methicillin-resistant Staphylococcus aureus (MRSA) isolates of bovine and human origin. A set of twenty-four MRSA isolates obtained from cattle (11), buffaloes (10) and their handlers (3) were subjected to PCR-ribotyping using primers specific for 16S-23S ribosomal spacer region. Polymorphism was observed with 23 ribotyp...

conclusions high hvr diversity among the studied mrsa isolates could be a result of insufficient or inadequate infection-control protocols in tabriz hospitals. moreover, the high number of hvr genotypes showed that hvr typing can be used along with other typing methods in epidemiological studies of mrsa as a useful tool for monitoring, tracking contaminations, and controlling infections in hosp...