Background & Aims: Today, different imaging techniques have been studied in the reading of radiationsensitive polymer gels dosage. Due to limitations of imaging techniques such as magnetic resonance imaging (MRI) and computed tomography (CT), ultrasound techniques are proposed for dose-dependent parameter extraction. In this study, using MAGIC-f (methacrylic and ascorbic acid in gelatin initiat...

BACKGROUND The AACC assembled a committee to identify and validate a standard creatine kinase MB isoenzyme (CK-MB) material to improve the comparability of CK-MB mass assays. METHODS Three protocols were used. In protocol I, various CK-MB materials prepared in different matrices were screened as candidate standards. In protocol II, participating manufacturers calibrated their systems with con...

We report an on-line coupling of gel permeation chromatography (GPC) to Fourier transform mass spectrometry (FTMS) using a modified commercial electrospray ionization (ESI) interface. Selected oligomer profiles for the sodiated (1+ through 5+ charge states) oligomer ions of a narrow-molecular-weight poly(methyl methacrylate) were generated and used for obtaining a calibration curve. Using the M...

Benzophenone-4 (BZ4) was separated from surfactants, dyes, preservatives, and other components of hair shampoos by thin-layer chromatography on silica gel 60 stationary phase, with ethyl acetate-ethanol-water-pH 6 phosphate buffer (15 : 7 : 5 : 1 v/v/v/v) as mobile phase. Densitometry scanning of chromatograms was performed at 285 nm. The densitometric calibration curve for BZ4 was nonlinear (s...

An easy and fast gel diffusion assay for detecting and monitoring lipase activity by quantification of fluorescein is described. By measuring the intensity of fluorescein, it is possible to obtain a calibration curve with a regression coefficient better than by using the radius of fluorescent haloes. Through the quantification of fluorescence intensity of fluorescein released after the hydrolys...

A fully automated procedure for the determination of rhodium has been developed using flow injection (FI) on-line microcolumn preconcentration coupled with electrothermal atomic absorption spectrometry (ETAAS). The proposed FI manifold and its operation make possible the introduction of the total eluate volume into the graphite atomizer, avoiding the necessity for optimisation of subsampling th...

Gel filtration of reduced protein polypeptide chains in 6 M guanidine hydrochloride has been evaluated as a means of estimating protein or protein subunit molecular weights as suggested by Davison (Science, 161, 906 (1968)). As a gel filtration medium, 6% agarose permits useful molecular weight estimates between the extreme limits of 80,000 and 1,000. Provided weight rather than volume is used ...