نتایج جستجو برای: fungal dna

تعداد نتایج: 556901  

2015
Iti Gontia-Mishra Niraj Tripathi Sharad Tiwari

A simple and rapid protocol for extracting high-quality DNA from filamentous fungi was studied. The method involved disruption of fungal cells by employing glass bead method, followed by inactivation of proteins using CTAB/proteinase K. The DNA yield from fungal isolates varied from 310-1879 μg g dry mycelium and a clear intact DNA band was observed upon agarose gel electrophoresis. Absorbency ...

Journal: :Scientific reports 2016
L Canfora E Malusà C Tkaczuk M Tartanus B H Łabanowska F Pinzari

A culture independent method based on qPCR was developed for the detection and quantification of two fungal inoculants in soil. The aim was to adapt a genotyping approach based on SSR (Simple Sequence Repeat) marker to a discriminating tracing of two different species of bioinoculants in soil, after their in-field release. Two entomopathogenic fungi, Beauveria bassiana and B. brongniartii, were...

2017
Huzefa A Raja Andrew N Miller Cedric J Pearce Nicholas H Oberlies

Fungi are morphologically, ecologically, metabolically, and phylogenetically diverse. They are known to produce numerous bioactive molecules, which makes them very useful for natural products researchers in their pursuit of discovering new chemical diversity with agricultural, industrial, and pharmaceutical applications. Despite their importance in natural products chemistry, identification of ...

Journal: :journal of mycology research 2015
golnaz sharafi ghasem vahedi ramak yahyaraeyat ali reza khosravi mohammad mehdi ranjbar

aspergillus fumigatus is a saprophyte fungus, widely spread in a variety of ecologicalniches and the most prevalent aspergilli responsible for human and animal invasiveaspergillosis. the first step to develop novel and efficient therapies is the identificationand understanding of the key tolerance and virulence factors of pathogens. the mainfocus of the present study is to perform the similarit...

Journal: :Journal of clinical microbiology 2000
C Martin D Roberts M van Der Weide R Rossau G Jannes T Smith M Maher

We report on a reverse-hybridization line probe assay (LiPA) which when combined with PCR amplification detects and identifies clinically significant fungal pathogens including Candida, Aspergillus, and Cryptococcus species. DNA probes have been designed from the internal transcribed-spacer (ITS) regions of Candida albicans, Candida parapsilosis, Candida glabrata, Candida tropicalis, Candida kr...

Journal: :Nucleic acids research 1974
S K Dutta

Several fungal species, representatives of all broad groups like basidiomycetes, ascomycetes and phycomycetes, were examined for the nature of repeated DNA sequences by DNA:DNA reassociation studies using hydroxyapatite chromatography. All of the fungal species tested contained 10-20% repeated DNA sequences. There are approximately 100-110 copies of repeated DNA sequences of approximately 4 x 1...

2017
Kasper Schei Ekaterina Avershina Torbjørn Øien Knut Rudi Turid Follestad Saideh Salamati Rønnaug Astri Ødegård

BACKGROUND The fungi in the gastrointestinal tract, the gut mycobiota, are now recognised as a significant part of the gut microbiota, and they may be important to human health. In contrast to the adult gut mycobiota, the establishment of the early gut mycobiota has never been described, and there is little knowledge about the fungal transfer from mother to offspring. METHODS In a prospective...

Journal: :Proceedings. Biological sciences 2017
Christopher C M Baker Dino J Martins Julianne N Pelaez Johan P J Billen Anne Pringle Megan E Frederickson Naomi E Pierce

Three ant species nest obligately in the swollen-thorn domatia of the African ant-plant Vachellia (Acacia) drepanolobium, a model system for the study of ant-defence mutualisms and species coexistence. Here we report on the characteristic fungal communities generated by these ant species in their domatia. First, we describe behavioural differences between the ant species when presented with a c...

Journal: :Fungal biology 2010
Lily Khadempour Sepideh Massoumi Alamouti Richard Hamelin Jörg Bohlmann Colette Breuil

The aim of this study was to develop DNA probes that could identify the major fungal species associated with mountain pine beetles (MPB). The beetles are closely associated with fungal species that include ophiostomatoid fungi that can be difficult to differentiate morphologically. The most frequently isolated associates are the pine pathogens Grosmannia clavigera and Leptographium longiclavatu...

Journal: :Applied and environmental microbiology 2016
Steven A Higgins Allana Welsh Luis H Orellana Konstantinos T Konstantinidis Joanne C Chee-Sanford Robert A Sanford Christopher W Schadt Frank E Löffler

UNLABELLED Members of the Fungi convert nitrate (NO3 (-)) and nitrite (NO2 (-)) to gaseous nitrous oxide (N2O) (denitrification), but the fungal contributions to N loss from soil remain uncertain. Cultivation-based methodologies that include antibiotics to selectively assess fungal activities have limitations, and complementary molecular approaches to assign denitrification potential to fungi a...

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