Abstract We report a series of ( Z )-2-acetyl-4-benzyliden-1-methyl-1 Н -imidazol-5(4 )-ones with pronounced solvent-dependent intensity fluorescence variation. The introduction the 2-acetyl group allows one to shift absorption and emission maxima long-wavelength region. have shown that these compounds can be used for staining endoplasmic reticulum fluorescent microscopy.

Abstract— We report two new analogs of the N871b fluorogen that contain CycloOctaTetraene. have studied photostability fluorescent complexes FAST protein with compounds by confocal microscopy when staining living HeLa Kyoto cells. One exhibits a higher in complex than previously published fluorogen. The results indicate cyclooctatetraene is highly promising compound for modification dyes based ...

Protein detection on SDS gels or on 2-D gels must combine several features, such as sensitivity, homogeneity from one protein to another, speed, low cost, and user-friendliness. For some applications, it is also interesting to have a nonfixing stain, so that proteins can be mobilized from the gel for further use (electroelution, blotting). We show here that coelectrophoretic staining by fluorop...

A direct staining procedure is described for fluorescence microscopy of fungal nuclei, chromosomes and mitochondria. The fluorochrome is a benzimidazol derivative (33258 Hoechst) known to bind selectively to deoxyribonucleic acid at neutral pH. The advantages of 33258 Hoechst relative to Feulgen compounds used previously to stain these structures include a greater intensity of fluorescence, th...

Fluorescence-labeled wheat germ agglutinin binds specifically to N-acetylglucosamine in the outer peptidoglycan layer of gram-positive bacteria. The peptidoglycan layer of gram-negative bacteria is covered by a membrane and is not labeled by the lectin. By exploiting this phenomenon, an alternative Gram staining technique has been developed.

We consider the physicochemical basis for enzyme-induced staining of cell membranes by fluorescent voltage-sensitive dyes, a method that may lead to selective labeling of genetically encoded nerve cells in brain for studies of neuronal signal processing. The approach relies on the induction of membrane binding by enzymatic conversion of a water-soluble precursor dye. We synthesized an amphiphil...

The fluorescein diacetate-ethidium bromide (FDA/EB) staining method for determining the viability of mycobacterial cells reported by Kvach and Veras was modified for applying the method to Mycobacterium leprae and Mycobacterium lepraemurium. Our method clearly differentiated green-stained cells from red-orange ones with either mycobacterial species and permitted their original fluorescence for ...

The fluorescent antibody staining technique was adapted by i\Ioody, Goldman, and Thomason (J. Bacteriol., 72, 357, 1956) for the identification of Mllalleomyces pseudomallei and has been used to identify additional microorganisms. This note reports the application of the direct fluorescent staining technique to the detection and identification of coagulase-positive staphylococci. Cowan (J. Path...

2,2,2-Trichloroethanol (TCE) incorporated into polyacrylamide gels before polymerization provides fluorescent visible detection of proteins in less than 5min of total processing time. The tryptophans in proteins undergo an ultraviolet light-induced reaction with trihalocompounds to produce fluorescence in the visible range so that the protein bands can be visualized on a 300-nm transilluminator...