This is the first report of the fluorescent antibody tissue stain for Treponema pallidum in the eye. The fluorescent antibody (FA) technique was first described in 1942 by Coons as a means of identifying pneumococcal antigens in tissue. It has subse­ quently been applied to identification of other bacteria and their antigens." The fluorescent antibody technique was applied to syphilis by Deacon...

Four stains for the detection of Pneumocystis carinii in respiratory specimens were compared for sensitivity, specificity, preparation time, and ease of interpretation. One hundred specimens were collected. Of these, 50 were induced sputum specimens and 50 were bronchoalveolar lavage fluid. All specimens were stained with Diff-Quik (DQ) (a modified Giemsa stain), a quick silver stain, and direc...

Paired nasopharyngeal aspirate (NPA) and midturbinate flocked-swab specimens from 153 children with respiratory symptoms were examined by the direct fluorescent antibody (DFA) technique. Seventy-four infants (49%) had a viral infection documented by DFA. The flocked-swab specimens had 93% sensitivity and 96.7% agreement with the NPA specimens, with a kappa coefficient of 93.4% (95% confidence i...

Four methods of detecting respiratory syncytial virus (RSV) from clinical specimens were evaluated. A total of 410 specimens consisting of nasopharyngeal washes, aspirates, and swabs were simultaneously tested for the presence of RSV by direct immunofluorescence assay (DFA), enzyme immunoassay (EIA) (Kallestad Pathfinder), shell vial centrifugation culture (SVC), and conventional culture. DFA i...

It is a particular advantage of the indirect fluorescent antibody (IFA) method that antibodies of different immunoglobulin classes may be separately detected and measured by the use of monospecific antiglobulin conjugates. Matuhasi, Mizuoka, and Usui (1966), Kiraly, Bachhausz, Jobbagy, Lajos, and Kovats (1968), Julian, Logan, and Norins (1969), and Atwood and Miller (1969) studied antibodies in...

The correlation between the modified counterimmunoelectrophoresis test and the indirect fluorescent-antibody test for detecting rabies virus antibodies in human sera was studied, using as a reference the standard mouse neutralization test. Higher sensitivity was observed with the counterimmunoelectrophoresis technique than with the indirect fluorescent-antibody method. The counterimmunoelectrop...

The correlation between the modified counterimmunoelectrophoresis test and the indirect fluorescent-antibody test for detecting rabies virus antibodies in human sera was studied, using as a reference the standard mouse neutralization test. Higher sensitivity was observed with the counterimmunoelectrophoresis technique than with the indirect fluorescent-antibody method. The counterimmunoelectrop...

rabies is acutely fatal encephalitis caused by a neurotropic virus. this virus belongs to the family ofrhabdoviridae and genus of lyssavirus. the virus is almost always transmitted to human through infectedmammalian saliva. rabies is inoculated to a wound by an infected animal bite. since infection is establishedin the cns, the outcome is almost always fatal. according to the outstanding role o...

Mice infected neonatally with homogenates from lymphomas induced by the Moloney virus showed a deficient or delayed antibody response, measured by the indirect fluorescent antibody test against Moloney lymphoma target cells in com parison with mice in.fected as adults. In neonatally infected mice there was a corre lation between the development of antibodies and the length of the preleukemic la...

No data are available concerning the seroprevalence of brucellosis in Central African Republic (CAR) and the last report concerning the seroprevalence of Q fever in CAR is from 1995. The aim of our study was to determine the prevalence of these diseases in CAR, especially in Zebu cattle. We used the Rose Bengal Plate Test to test 2032 bovine serum samples for antibodies to Brucella spp. and an ...