A simple procedure is described for the mechanical isolation of protoplasts of unfertilized and fertilized barley egg cells from dissected ovules. Viable protoplasts were isolated from ~75% of the dissected ovules. Unfertilized protoplasts did not divide, whereas almost all fertilized protoplasts developed into microcalli. These degenerated when grown in medium only. When cocultivated with barl...

The endosymbiotic bacterium Wolbachia pipientis manipulates host reproduction by rendering infected males reproductively incompatible with uninfected females (cytoplasmic incompatibility; CI). CI is believed to occur as a result of Wolbachia-induced modifications to sperm during maturation, which prevent infected sperm from initiating successful zygote development when fertilizing uninfected fe...

Observations in this laboratory have indicated that human subjects with malignant growths exhibit a significant elevation in plasma glutamic acid concentration (2, 5) . No such elevation was observed in subjects with benign growths, al though it has been shown that an elevated plasma glutamic acid level may be observed in clinical conditions other than malignancy (5). The results suggest that p...

Contraception may provide a useful nonlethal management tool when it is desirable to reduce populations of birds. We tested the efficacy of 20,25 diazacholesterol, and immunization with avian gonadotropin-releasing hormone (AGnRH-I) and chicken riboflavin carrier protein (cRCP) as contraceptives and investigated their modes of action in Coturnix quail (Coturnix coturnix japonica). Females that ...

The current studies were undertaken to assess the ability of humoral immune response in breeding hens and the protective immunity provided by administration of purified Salmonella gallinarum (SG) porins to the progeny. Two hundred and ten broiler breeder hens, 53 weeks old, were divided into three groups and subcutaneously immunized via multiple sites at 0 and 10 days with either: a) 10 μg SG p...

Adult Northern bobwhite were used to test the hypothesis that dietary methionine levels recommended by the NRC for breeding quail are excessive for wild bobwhite. We tested the hypothesis by comparing immunocompetence, reproductive performance, and chick viability of Northern bobwhite hens fed diets containing low (0.31%), moderate (0.39%), or high (0.47%) concentrations of methionine. Chick vi...

Abstract The objective of this study was to investigate the effects in ovo injection sodium borate on hatching power, chick weight, length fertile broiler eggs. A total 256 eggs were incubated study. On Day 18 incubation, two groups injected with 0.1 mL 0.5 mg and 1 dissolved saline, used as sham control (injected saline) uninjected control. Hatching power apparently increased ( P ≤ 0.05) by (0...

We studied the effect of dietary source (organic or inorganic) and level of Se on the Se uptake of chick embryos. After receiving a low-Se diet for 16 wk, 126 Leghorn laying hens were randomly assigned to one of seven dietary treatments. Treatments consisted of feeding a low-Se basal diet alone or with one of three levels of added Se (0.1,0.2, or 0.3 mg/kg Se) supplied by sodium selenite or Se-...

The eggs of the zebra fish Brachydanio rerio (Hamilton-Buchanan) were used in these studies. Roosen-Runge (1936, 1938, 1939), Marrable (1959,1962a) and others have described various aspects of the biology of this fish when maintained in the laboratory. It is a freshwater tropical teleost, the females laying nonsticky, non-pigmented demersal eggs which undergo a regular and predictable pattern o...