Previous observations obtained from a culture of blastodermal cells on a mouse fibroblast feeder layer (STO) suggested that STO cells provide a factor or factors that facilitate development of avian primordial germ cells (PGC) from dispersed embryo cells. The purpose of the current study was to test the hypothesis that soluble factors produced by STO cells are responsible, at least in part, in ...

objective(s): ex vivo expansion of hematopoitic stem cells is an alternative way to increase umbilical cord blood (ucb)-cd34+ cells for bone marrow transplantation. for this purpose demineralized bone matrix (dbm) and mineralized bone allograft (mba) as two scaffolds based on bone matrix and stem cell niche, were simultaneously used to enhance the effect of human mesenchymal progenitor cells (m...

We describe a WDM-based optical access network architecture for providing broadband Internet services. The architecture uses a passive collection and distribution network and a configurable Feeder network. Unlike earlier papers that concentrate on the physical layer design of the network, we focus on higher layer architectural considerations. In particular we discuss the joint design of the ele...

background: blood sucking mites are important avian ectoparasites which being found on bird species worldwide. their presence are problematic for the producers either through potential direct effects on weight gain, egg produc­tion, sperm production in roosters or as nuisance pests on worker handle hens and eggs. the aim of this study was pointing out of the status of haematophagus mites. metho...

There is a great interest in using epithelium generated in vitro for tissue bioengineering. Mouse 3T3 fibroblasts have been used as a feeder layer to cultivate human epithelia including corneal epithelial cells for more than 3 decades. To avoid the use of xeno-components, we evaluated human fibroblasts as an alternative feeder supporting human corneal epithelial regeneration. Five human fibrobl...

  　 Objective(s): Ex vivo expansion of hematopoitic stem cells is an alternative way to increase umbilical cord blood (UCB)-CD34+ cells for bone marrow transplantation. For this purpose demineralized bone matrix (DBM) and mineralized bone allograft (MBA) as two scaffolds based on bone matrix and stem cell niche, were simultaneously used to enhance the effect of human mesenchymal pro...

Patient-specific human induced pluripotent stem (hiPS) cells not only provide a promising tool for cellular disease models in general, but also open up the opportunity to establish cell-type-specific systems for personalized medicine. One of the crucial prerequisites for these strategies, however, is a fast and efficient reprogramming strategy from easy accessible somatic cell populations. Kera...

Epithelial-mesenchymal interactions control epidermal growth and differentiation, but little is known about the mechanisms of this interaction. We have examined the effects of human dermal microvascular endothelial cells (DMEC) and fibroblasts on keratinocytes in conventional (feeder layer) and organotypic cocultures (lifted collagen gels) and demonstrated the induction of paracrine growth fact...