Cytochrome P450 (P450) assays use probe substrates to interrogate the influence of new chemical entities toward P450 enzymes. We report the synthesis and study of a family of bioluminogenic luciferin acetal substrates that are oxidized by P450 enzymes to form luciferase substrates. The luciferin acetals were screened against a panel of purified P450 enzymes. In particular, one proluciferin acet...

BACKGROUND Real-time assays for Yellow fever virus (YFV) would help to improve acute diagnostics in outbreak investigations. OBJECTIVES To develop a real-time assay for YFV able to detect African and South American strains. STUDY DESIGN Three short probe (14-18 nt) formats were compared and a plasmid-transcribed RNA standard was used to test the performance of the assays. Additionally the n...

The present study describes the phytochemical investigations of the crude extracts of rhizomes and leaves of Geranium wallichianum. The crude extracts were fractionated to obtain n-hexane, ethyl acetate, and n-butanol fractions, which were subjected to different biological activities and enzyme inhibition assays to explore the therapeutic potential of this medicinally important herb. The result...

Coupled enzyme assays are described for measuring inorganic phosphates, organic phosphates and phosphate-liberating enzymes in biological material. The assays all determine Pi by its reaction with inosine, catalysed by nucleoside phosphorylase; this yields ribose 1-phosphate and hypoxanthine. The hypoxanthine is oxidized to uric acid by xanthine oxidase, and may be measured either by the absorb...

Etravirine (ETR) is a second-generation nonnucleoside reverse transcriptase (RT) inhibitor (NNRTI) active against common human immunodeficiency virus type 1 (HIV-1) drug-resistant strains. This study was designed to determine the extent to which each of the Y181C or G190A mutations in RT might confer resistance to ETR and other members of the NNRTI family of drugs. Recombinant HIV-1 RT enzymes ...

Abstract The current work examined the therapeutic potential of 6-aminoflavone (6AF) against mouse model-based oxidative stress-driven synaptic and memory impairment caused by lipopolysaccharides (LPSs). In brains experimental mice, LPS administration for 3 weeks significantly increased stress inhibiting antioxidant enzymes, including superoxide dismutase, peroxidase, catalase, glutathione, upr...

In this study, combining the nanoparticle embedded sensors with lateral flow assays, a novel strategy for ensuring the quality of signalling in lateral flow assays (LFAs) was developed. A LFA for reactive oxygen species (ROS) is reported that is based on horse radish peroxidase (HRP) which is co-entrapped with Texas Red dextran inside porous polyacrylamide nanoparticles. In this system, enzymes...

potato tuberworm, phthorimaea operculella zeller (lepidoptera; gelechiidae) is a worldwide pest of solanaceous crops. larvae feed inside galleries in foliage, stems and tubers making chemical control unsuccessful, so other control methods should be applied. in recent years many plants have received genes that encode toxic proteins as a strategy to resist insect pests. in this study, optimal ph ...

Since 2004, unspecific peroxygenases, in short UPOs (EC. 1.11.2.1), have been explored. are closing a gap between P450 monooxygenases and chloroperoxidases. These enzymes highly active biocatalysts for the selective oxyfunctionalisation of C–H, C=C C-C bonds. secreted fungal proteins Komagataella phaffii (Pichia pastoris) is an ideal host high throughput screening approaches UPO production. Het...

Obesity is a chronic disease affecting both adults and children worldwide. One major cause of obesity high-calorie intake due to overconsumption foods rich in fat carbohydrates. Hence, can be controlled by controlling the diet other lifestyle changes that increase energy expenditure. However, this not always possible for individuals who are already overweight suffering from diseases. Therefore,...