Cellulases are being widely employed in lignocellulosic biorefineries for the sustainable production of value-added bioproducts. However, high cost, sensitivity, and non-reusability free cellulase enzymes impede their commercial applications. Enzyme immobilization seems to be a potential approach address aforesaid complications. The current study aims at recombinant endoglucanase (CelA) origina...

Background. Thiamine kinase is a medical enzyme, genetically determined "breakdowns" during the synthesis of which lead to number neurodegenerative diseases. Knowledge distribution enzyme in compartments brain, regulatory capabilities globule formation coenzyme form vitamin B1 – thiamine diphosphate, will enable perform targeted correction pathological conditions. Purpose study. To determine lo...

drinking water has received considerable attention recently. however, misuse and mismanagement have resulted in a rapid and widespread decline in source-water quality and supply. water quality guidelines can be used to identify constituents of concern in water, to determine the levels to which the constituents of water must be treated for drinking purposes. membrane technology for the water cyc...

Electrospun polymer membranes are regarded as prospective biosensor components due to their large specific surface area and diverse opportunities for chemical modifications. However, intricate porous structure can impede diffusion render some analyte-binding sites inaccessible. To overcome these limitations improve analyte adsorption onto the polymer, a pressure-driven sample flow through membr...

The present study was undertaken to localize adenylate cyclase activity in salivary glands by cytochemical means. For the study, serous parotid glands and mixed sublingual glands of the rat were used. Pieces of the fixed glands were incubated with adenosine triphosphate (ATP) or adenylyl-imidodiphosphate (AMP-PNP) as substrate: inorganic pyrophosphate or PNP liberated upon the action of adenyla...

Freeze-thawing of microsome-enriched membranes from living bark tissues of black locust trees, especially those from less hardy tissues, caused a drastic increase in sensitivity to Ca(2+) and a complete loss of the regulatory action of Mg(2+) in membrane-bound phospholipase D activity with endogenous (membrane-bound) substrates. Also, the freeze-thaw cycle made phospholipase D in these membrane...

Studies of mitochondrial biogenesis in yeast have been hampered by a lack of suitable membrane markers in anaerobically grown cells subsequently grown in air. Cytochrome c peroxidase activity and subcellular location was studied to determine whether it would be a useful marker for an analysis of mitochondrial formation. Cytochemical tests revealed enzyme reaction product on all mitochondrial me...

In Dictyostelium discoideum, the lysosomal enzyme alpha-mannosidase is first synthesized as an N-glycosylated precursor of Mr 140,000. After a 20-30-min lag period, up to 30% of the precursor molecules are rapidly secreted, whereas the rest remain cellular and are proteolytically processed (t 1/2 = 8 min) to mature subunits of Mr 58,000 and 60,000. The secreted precursor is modified more extens...

An endoglycosidase is described in isolated liver plasma membranes that brings about a rapid and selective degradation of membrane-associated heparan sulphate, pre-labelled biosynthetically with Na2(35)SO4. The enzyme attacked mainly the polysaccharide chains of a hydrophobic membrane proteoglycan and it had little effect on a proteoglycan that could be displaced from the membranes with 1.0 M-N...

The effects of temperature and of the action of a purified phospholipase C enzyme preparation on human red blood cell membranes has been investigated by chemical analyses, circular dichroism, and proton magnetic resonance measurements. The results indicate that a substantial fraction of the phospholipids and the proteins of the membranes can change structure independently of one another, sugges...