An aseptic meningitis outbreak occurred during a period from February to May 2004 in São Joaquim da Barra, a town in the northern region of São Paulo State. A total of 40 cases were reported to the Epidemiological Surveillance Center of São Paulo State. Cerebrospinal fluid samples obtained from 23 patients were sent to the Adolfo Lutz Institute for isolation of the virus. These samples were ino...

Polypeptide 2C is essential for picornavirus replication. Although many data on multiple functions of this highly conserved protein are available, the mechanism of RNA binding is still obscure. In this work, protein 2C of echovirus-9 strain Barty was expressed as a histidine-tagged protein in E. coli followed by nondenaturing purification to homogeneity. After incubation of 2C protein with diff...

Studies of the effects of temperature and salinity on the survival of three enteric viruses (poliomyelitis type 1, echovirus-6, and coxsackievirus B-5) under controlled laboratory conditions and in situ indicate that temperature rather than salinity is the critical factor affecting their stability, in that the higher the temperature the more rapid was the loss of viral infectivity. In the labor...

Masae Iwai*, Eiji Horimoto, Mayumi Obara, Masatsugu Obuchi, Takeshi Kurata, Kumiko Kawagoshi, Sumika Nakamura, Hiroyuki Shimizu, Hiromu Yoshida, and Takenori Takizawa Department of Virology, Toyama Institute of Health, Toyama 939-0363; Tonami Health Center, Toyama 939-1506; Takaoka Health Center, Toyama 933-8523; and Department of Virology II, National Institute of Infectious Diseases, Tokyo 20...

An outbreak of aseptic meningitis has been ongoing in several areas of Latgale region, in the south-eastern part of Latvia since the end of June 2010. By 9 August 2010, 114 cases had been notified. Most of the cases were registered in the city and amalgamated municipality of Daugavpils and neighbouring territories. According to current evidence, two types of enteroviruses, coxsackie A-9 virus a...

Antibody prepared against HEp-2 cells inhibited the replication of echovirus I. The number of infective centres was reduced and the cells which were infected produced less virus per cell than control cells. The attachment of virus to cells was not affected. The inhibition was reduced by increasing the multiplicity of infection. Application of antibody 5 min. after virus adsorption at 4 ° reduce...

Despite the fact that enteroviruses are implicated in a variety of human diseases, there is no approved therapy for the treatment of enteroviral infections. Here, a series of 2,6-dihalophenyl-substituted 1H,3H-thiazolo[3,4-a]benzimidazoles with anti-enterovirus activity is reported. The compounds elicit potent activity against coxsackievirus A9, echovirus 9 and 11 and all six strains of coxsack...

Receptor activities of HeLa cells were evaluated for ability to both attach and eclipse enteroviruses after exposure of cells to acid or heat. A modified procedure of acid (pH 1.5) elution of cell-associated virus, as compared with other procedures, provided a general method for the optimal recovery of receptor-bound enteroviruses. With this procedure, eclipse of virus operationally was conside...

BACKGROUND & OBJECTIVES Echovirus 11 (ECV11) is one of the most frequent non-polio enteroviruses isolated from stool samples of children with acute flaccid paralysis in north India. The present work was undertaken to study the sequence variability in the 440 bp of 5'-non-translated region of ECV11 genome using heteroduplex mobility assay (HMA). METHODS Twelve ECV11 isolates were studied for s...