نتایج جستجو برای: dithiothreitol
تعداد نتایج: 3478 فیلتر نتایج به سال:
A newly introduced sulphydryl compound, dithiothreitol (DTT), is evaluated for its optimal conditions of inactivation of IgM antibodies. The maximal effects of DTT reagent are observed when its final concentrations are between 0.0025 M and 0.005 M, pH between 7.0 and 8.0, and incubation at 37 degrees C. Concentrations over 0.01 M, pH values greater than 8.0, and an incubation temperature over 4...
In comparing the use of the sulphydryl compounds 2-mercaptoethanol (2ME) and dithiothreitol (DTT) a rapid one-stage technique for titrating IgG anti-A and anti-B using DTT in an antiglobulin test was tried. The results correlated well with those obtained by either of the two-stage methods using 2ME or DTT.
Atmospheric quinoid polycyclic aromatic hydrocarbons (PAHQs) have adverse health effects as redox-active species in particulate matter (PM). Several PAHQs are known to be very reactive the dithiothreitol (DTT) assay; however, it is unclear if pyrenequinones, their parent pyrene one of most abundant (PAHs) atmosphere, contribute loss DTT PM extracts. Herein, by employing assay, we evaluated oxid...
Hydroxyl radicals were generated by pulse radiolysis of N20-saturated aqueous solutions. The O H radicals react with thiols such as 1,4-dithiothreitol by abstracting S-bound H-atoms (k = 1.5 x 101() dm3 m ol“ 1 s_l). The 1,4-dithiothreitol radical HSCH2(C H O H )2CH2S' (2) closes the ring (k — 1.5 x 106 s“1) thereby forming the three-electron-bonded species Q H 2(C H O H )2C H 2S— $ H (3) in eq...
In contrast to the F1-ATPases from bovine mitochondria and the thermophilic Bacillus PS3, which are reversibly inhibited by dequalinium in the absence of irradiation, the Mg2+-ATPase activity of heat- or dithiothreitol-activated chloroplast F1 (CF1) from spinach chloroplasts is slightly stimulated by dequalinium. Conversely, dequalinium is a partial inhibitor (maximal inhibition is 85-90%) of t...
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