We have previously shown that heat acclimation provides protection against central nervous system oxygen toxicity (CNS-OT). This was well correlated with increased levels of heat shock protein 72 (HSP72). We now examine other antioxidative defenses against CNS-OT that are correlated with heat acclimation. Two groups of male Sprague-Dawley rats were used. The heat-acclimated group (HA) was expos...

The culture system of bone marrow mesenchymal stem cells (bmMSCs) in the normoxic environment does not imitate hypoxic milieu typical biological conditions, thus conditions may improve survival, and growth attributes bmMSCs during vitro culture. Therefore, present study was conducted at ICAR-CIRG, Makhdoom year 2020 with objective to investigate changes characteristics cultured caprine (cbmMSCs...

INTRODUCTION This study was conducted to evaluate the oxidant/antioxidant balance (oxidative stress status) and plasma essential trace element levels in patients with bronchial asthma or allergic rhinitis. METHODS A total of 94 individuals consisting of 19 allergic asthmatics; 17 non-allergic asthmatics; 22 patients with allergic rhinitis; and 36 healthy control people were enrolled into this...

PURPOSE To establish a method for morphometric analysis of retrogradely labeled retinal ganglion cells (RGCs) of the mouse retina, to be used for the study of molecular aspects of RGC survival and neuroprotection in this model; to evaluate the effect of overexpression of Cu-Zn-superoxide dismutase (CuZnSOD) on RGC survival after severe crush injury to the optic nerve, and to assess the effect o...

sulfur mustard (sm) has been identified as an important chemical weapon. during the iran-iraq war of 1980-88, the extensive usage of sm against iranian civilians and military forces was proven. this agent has been shown to cause severe damage mainly in the skin, eyes, lungs,  and  respiratory  tract  in  iranian  veterans.  the  most  common  disease  is bronchiolitis obliterans (bo)). sm incre...

The sensitivity of copper,zinc (CuZn)- and manganese (Mn)-superoxide dismutase (SOD) to exogenous estradiol benzoate (EB) was investigated in Wistar rats during postnatal brain development. Enzyme activities were measured in samples prepared from brains of rats of both sexes and various ages between 0 and 75 days, treated sc with 0.5 micro g EB/100 g body weight in 0.1 ml olive oil/100 g body w...

Copper-zinc superoxide dismutase (CuZnSOD) acquires its catalytic copper ion through interaction with another polypeptide termed the copper chaperone for SOD. Here, we combine X-ray crystallographic and analytical ultracentrifugation methods to characterize rigorously both truncated and full-length forms of apo-LYS7, the yeast copper chaperone for SOD. The 1.55 A crystal structure of LYS7 domai...

pies after ethanol/chloroform treatVu/us cells ment (for CUZnSOD assay) or for sam20 0.0053 0.0037 0.0027 0.0072 0.0070 0.0050 plea from tissues that contain a trace 0.0144 0.0121 0.0100 0.0179 0.0121 0.0102 amount of xanthine oxidase (EC 1.2.3.2) activity. Now, we have modifled the method by simply substituting 1 minolfL diethylenetriamine-pentaacetic acid (DETAPAC) for 0.1 mmoIJL EDTA and add...

Superoxide dismutases (SODs) are metalloenzymes that play a primary role in the protection against oxidative stress in plants and other organisms. We have characterized four SOD genes in Lotus japonicus and have analyzed their expression in roots and four developmental stages of nodules. The expression of cytosolic CuZnSOD, at the mRNA, protein, and enzyme activity levels, decreases with nodule...

Received 21 May 1996 and in revised form 2 September 1996 Accepted 9 September 1996 Background Amyotrophic lateral sclerosis (ALS) is a fatal disease in which degeneration of upper and lower motor neurons leads to progressive weakness of bulbar, limb, thoracic, and abdominal muscles with relative sparing of oculomotor muscles and sphincter function. Although the clinical manifestations and path...