O Objective: To identify the use of traditional food, quantify main foods consumed in region, and propose a dish good eating for Totonac region. Methodology: A semi-structured survey was carried out 328 households municipality Filomeno Mata, Veracruz, Mexico. The Household Dietary Diversity Score (HDDS) section were used to obtain information about regional that local population consumes on dai...

Suspension culture of single adult rat flexor digitorum brevis (FDB) muscle fibres in Vitrogen, a purified collagen, on tissue culture plastic or glass with mesh ring supports is superior to culture upon other substrates including collagen-, laminin-, or Vitrogen-coated tissue culture plastic. The Vitrogen gel-fibre mixture which attaches to glass or plastic provides at least 10 times more fibr...

Tissue engineering is a rapidly advancing technology in the field of regenerative medicine. For the transplantation of cell sheets, a carrier must maintain the shape of a cell sheet from a culture dish to affected sites as well as release the sheet easily onto the lesion. In this study, we examined the utility of a novel, poly(lactic acid)-based carrier for cell sheets transplantation to the co...

Bone marrow stromal cells taken from EGFP transgenic mice were sorted by magnetic beads with surface markers for Sca-1 and Thy-1. The cells were then co-cultured on organotypic hippocampal slice or with neuronal cell feeder in dish. On hippocampus, both Sca-1 and Thy-1 positive cells showed 4- 8 folds higher potential to show neuron-like morphology than negative cells. In dish, negative cells f...

Periodical contraction of a cultured muscle tube has been measured with laser in vitro. C2C12 (mouse myoblast cell line) was cultured with High-glucose Dulbecco’s Modified Eagle’s Medium on a dish to make muscle tubes. Differentiation from myoblasts to myotubes was induced with an additional horse serum. Repetitive contraction of the tube was generated by electric pulses lower than sixty volts ...

Inbred male Lewis (LEW) and Brown Norway (BN) rats were used for experiments. Plastic dish nonadherent bone marrow cells (BMC) were prepared and cultured with 2% PWM-SCM for 6 days in a fibrin clot culture system to detect colony-forming units in culture (CFU-C). Clusters (10 to 50 cells per aggregate) and colonies (>50 cells per aggregate) in cultures were counted as CFU-C. LEW-derived CFU-C w...

Development of scaffolds consisting of micro-/nano-sized materials have attracted a great deal of attention for their potential use in tissue engineering tools. We prepared a cell culture scaffold of carbon nanotubes, which is a typical bio-inert nanomaterial, and then investigated the surface morphology and properties. A three-dimensional nano-level network structure was observed using a scann...

Sichuan cuisine is one of the eight major cuisines in China. It draws on strengths many families and forms characteristics “one dish, style, hundreds dishes”. The culture sichuan extensive profound. Under background construction ' Belt Road strategy going out Chinese culture, it particularly important to understand translate cultural connotation order make translation more faithful reflect char...

This paper examines the efficacy of liver cancer treatment using HA22T cancer cells specific to the Taiwanese population. The Clonogenic Assay is the current standard method for detecting liver cancer. This paper uses image processing technology and a fuzzy inference system to identify in-vitro colonies of cancer cells. A scanner was first used to capture an image of the culture dish. This imag...

A novel cover slip-transfer culture system was designed to study the functional roles of stromal cells in hemopoiesis, particularly erythropoiesis. Human bone marrow stromal cell colonies were allowed to develop on small glass cover slips in liquid medium. The cover slips, along with the stromal cell colonies and progenitors attached to them were then transferred to a new tissue culture dish an...