Calf lens protein components separated by DEAE chromatography have been related to the crystallins isolated by the new method. Gamma crystallin is the first fraction eluted from the column, folloioed by the beta crystallin components, and then the alpha crystallin subgroup. Since the DEAE separable components could thus be identified as members of the alpha, beta, or gamma crystallin subgroups,...

The interaction of the cationic carbocyanine dye Stains-all (1-ethyl-2-[3-(1-ethyl-naphthol[1,2-d]thiazolin-2-ylidene)-2- methylpropenyl]naphthol[1,2-d]thiazolium bromide) with the eye lens proteins crystallins has been studied. alpha- and gamma-crystallins do not bind the dye, while beta- and delta-crystallins do, consistent with the fact that the latter two proteins bind the calcium ion. beta...

the purpose of this investigation was to study and compare the electrophoretic patterns of optic lensproteins of different species of domestic animals at pre- and post-natal ages. optic lenses were removed from the embryo or adult sheep, cattle, goat, camel and chicken at the slaughter-house then homogenized and subjected to sodium dodecylsulfate polyacrylamide gel electrophoresis (sds-page). i...

and patterns of expression, γ-crystallins have generally appeared to be the most lens-specific of the crystallins. Here we examine the mouse γS-crystallin (γS) gene and its expression. Methods: The cDNA and gene for mouse γS were cloned and sequenced. The Crygs gene was mapped using genetic crosses. Expression patterns in mouse and cow were examined by northern blot, PCR and western blot using ...

PURPOSE To determine the age-related and the cataract-specific changes in the crystallin composition in lenses of accelerated-senescence OXYS (cataract model) and Wistar (control) rats. METHODS The water soluble (WS) and insoluble (WIS) fractions of the lens proteins were separated; the identity and relative abundance of each crystallin in WS fraction were determined with the use of two-dimen...

A high glucose concentration in vivo or an increased glucose of glucose 6-phosphate concentration in vitro has been found to lead to the glycosylation of epsilon-amino groups of lysine residues in bovine and rat lens crystallins. In vitro, this glycosylation imparts an increased susceptibility of the crystallins to sulfhydryl oxidation. Disulfide crosslinks result in the formation of high molec...

Alpha, beta, and gamma crystallins from rabbit eye lens have been prepared by continuousflow paper electrophoresis and gel filtration. These methods yielded well-defined fractions in a highly reproducible manner with essentially quantitative recovery of material. The behavior of each of the crystallins in diethylaminoethyl cellulose has been examined. Sulfhydryl contents of 2.9, 6.1, and 26.0 m...

The crystallins in the lenses of ICR/f mutation rat, a known hereditary cataract model, were analyzed during cataractogenesis. Opacification of the mutant lenses was found to be accompanied by changes in crystallin structure and composition, including several deletions of the N-terminals of beta-crystallins and low molecular weight alpha- crystallins. Because similar deletions were observed whe...

Taxon specific lens crystallins in vertebrates are either similar or identical with various metabolic enzymes. These bifunctional crystallins serve as structural protein in lens along with their catalytic role. In the present study, we have partially purified and characterized lens crystallin from Indian spiny-tailed lizard (Uromastyx hardwickii). We have found lactate dehydrogenase (LDH) activ...