Metal cutting is a severe plastic deformation process involving large strains, high strain rates, and high temperatures. Conventional analysis of the chip formation process is based on bulk material deformation disregarding the inhomogeneous nature of the material microstructure. A series of orthogonal cutting tests of AISI 1045 and 1144 steel were conducted which yielded similar process charac...

Experiments are performed to correlate and model CHF during subcooled flow boiling of dielectric fluoro~rbon (FC-72) liquid on a smooth 12.7 x 12.7 mm* sirn~at~ electronic chip inside a vertical rectangular channel. Two distinct CHF regimes are established based on flow visualization and experimental data. At low flow velocities, CHF is accompanied by the formation of a continuous vapor blanket...

The Hsp70-interacting E3-ubiquitin ligase CHIP has been implicated in the decision as to whether a target protein enters the refolding or the degradation pathway. To further characterize the activity of CHIP we purified untagged Homo sapiens and Drosophila melanogaster CHIP (hCHIP, dCHIP). In contrast to other E3-ubiquitin ligases, both hCHIP and dCHIP proteins formed homodimers at physiologica...

The conventional hanging drop technique is the most widely used method for embryoid body (EB) formation. However, this method is labor intensive and limited by the difficulty in exchanging the medium. Here, we report a microfluidic chip-based approach for high-throughput formation of EBs. The device consists of microfluidic channels with 6 × 12 opening wells in PDMS supported by a glass substra...

CHIP is a dimeric U box E3 ubiquitin ligase that binds Hsp90 and/or Hsp70 via its TPR-domain, facilitating ubiquitylation of chaperone bound client proteins. We have determined the crystal structure of CHIP bound to an Hsp90 C-terminal decapeptide. The structure explains how CHIP associates with either chaperone type and reveals an unusual asymmetric homodimer in which the protomers adopt radic...

Measurements of chloride concentration are important in a range of applications. We report here on our progress toward the fabrication and testing of small, inexpensive chloride sensors integrated with sensing electronics on a CMOS chip. We describe and demonstrate a process for formation of gold and silver chloride/ silver electrodes on a CMOS chip. Cyclic voltammetry is performed on chip-scal...

This paper reports a novel cell culture chip, namely “inverting microwell array chip”, for cultivation of human induced pluripotent stem cells with controlled microenvironment. Our “inverting microwell array chip” comprises a lower hydrogel microwell array and an upper polystyrene (PS) culture surface. We demonstrate the formation of uniform human induced pluripotent stem cell aggregates in the...