Plant parasitic nematodes comprise several groups; the most economically damaging of these are the sedentary endoparasites. Sedentary endoparasitic nematodes are obligate biotrophs and modify host root tissue, using a suite of effector proteins, to create a feeding site that is their sole source of nutrition. They feed by withdrawing host cell assimilate from the feeding site though a structure...

Plant-parasitic cyst nematodes synthesize and secrete effector proteins that are essential for parasitism. One such protein is the 10A07 effector from the sugar beet cyst nematode, Heterodera schachtii, which is exclusively expressed in the nematode dorsal gland cell during all nematode parasitic stages. Overexpression of H. schachtii 10A07 in Arabidopsis thaliana produced a hypersusceptible ph...

Plant parasitic nematodes need to overcome the barrier presented by plant cell wall in order invade their host. A variety of degrading enzymes are present endoparasitic including that degrade cellulose (beta 1,4 endoglucanases) and various pectin components. We describe cloning functional analysis genes encoding GH53 arabinogalactan endo-1,4-beta-galactosidases from three related Globodera rost...

Title of thesis: PHYSIOLOGICAL AND MOLECULAR STUDIES OF ETHYLENE EFFECTS ON SOYBEAN ROOT INFECTION BY SOYBEAN CYST NEMATODES Ping Xue, Master of Science, 2007 Directed By: Dr. Theophanes Solomos, Department of Plant Science and Landscape Architecture Soybean cyst nematode (SCN), Heterodera glycines, is one of the most devastating pests of soybean in the world. Several earlier reports demonstrat...

Summary Selected strains of Pasteuria species from the endospore-forming Gram-positive bacteria group have potential to be developed into control agents for plant-parasitic nematodes. If is deployed successfully as a agent, endospores bacterium initially adhere cuticle infective juvenile. Studies isolated root-knot nematodes suggested that collagen-like fibres on endospore surface interact with...

SUMMARY Transgenic tobacco and Arabidopsis thaliana carrying the Arabidopsis endo-1,4-beta-glucanase (EC 3.2.1.4) Cel1 promoter fused to the beta-glucuronidase (GUS) reporter gene were infected with the root-knot nematode, Meloidogyne incognita, and either the tobacco cyst nematode, Globodera tabacum (tobacco), or beet cyst nematode, Heterodera schachtii (Arabidopsis). Cel1-driven GUS expressio...

Linalool is either a toxic compound to a few species of plant parasitic nematodes or attractive to entomopathogenic nematodes. This compound is produced and emitted by several host plants of Globodera rostochiensis and G. pallida, the potato cyst nematodes (PCN). With the aim to reveal the effect of linalool on PCN, laboratory assays were carried out. Survival of PCN second-stage juveniles (J2s...

Roundworms of the Nematoda comprise one of the largest animal phyla on Earth (1). They inhabit diverse terrestrial and aquatic niches through adaptations of a spectrum of trophic groups, including parasites that threaten human, animal and crop plant health. The most well-known nematode, Caenorhabditis elegans, is a native soil-dwelling microbivore that has emerged as a premier model for animal ...

Nematodes that parasitize plant roots cause huge economic losses and have few mechanisms for control. Many parasitic nematodes infect plants by reprogramming root development to drive the formation of feeding structures. How nematodes take control of plant development is largely unknown. Here, we identify two host factors involved in the function of a receptor ligand mimic, GrCLE1, secreted by ...

Recent advances in molecular biology have enabled the genotyping of individual nematodes, facilitating the analysis of genetic variability within and among plant-pathogenic nematode isolates. This review first describes representative examples of how RFLP, RAPD, AFLP, and DNA sequence analysis have been employed to describe populations of several phytonematodes, including the pinewood, burrowin...